Sonic hedgehog stimulates proliferation of human periodontal ligament stem cells

C. Martínez; P. C. Smith; J. P. Rodriguez; V. Palma

doi:10.1177/0022034510391797

Sonic hedgehog stimulates proliferation of human periodontal ligament stem cells

C. Martínez, P. C. Smith, J. P. Rodriguez, V. Palma^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Regulation of cell renewal in the periodontium is a critical cell function that has not been clarified. Sonic hedgehog (Shh) is a secreted signaling molecule that plays a key role during development and adult tissue homeostasis. In the present study, we have analyzed the role played by Shh in human periodontal ligament stem cell (HPLSC) proliferation. HPLSC were isolated with anti-STRO-1 antibodies. Shh increased the expression of GLI1 and PTC-1 and selectively stimulated cell proliferation in STRO-1(+) derived from adult periodontal ligament. Shh components were localized to primary cilia in STRO-1(+) cells after Shh stimulation. STRO-1(+) also expressed Shh, suggesting an autocrine-regulated phenomenon. Thus, we propose that Shh plays a critical role in the regulation of cell proliferation in STRO-1(+)/HPLSC.

Original language	English
Pages (from-to)	483-488
Number of pages	6
Journal	Journal of Dental Research
Volume	90
Issue number	4
DOIs	https://doi.org/10.1177/0022034510391797
State	Published - Apr 2011
Externally published	Yes

Bibliographical note

Keywords

human periodontal ligament stem cells
primary cilia
proliferation
Sonic hedgehog

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10.1177/0022034510391797

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title = "Sonic hedgehog stimulates proliferation of human periodontal ligament stem cells",

abstract = "Regulation of cell renewal in the periodontium is a critical cell function that has not been clarified. Sonic hedgehog (Shh) is a secreted signaling molecule that plays a key role during development and adult tissue homeostasis. In the present study, we have analyzed the role played by Shh in human periodontal ligament stem cell (HPLSC) proliferation. HPLSC were isolated with anti-STRO-1 antibodies. Shh increased the expression of GLI1 and PTC-1 and selectively stimulated cell proliferation in STRO-1(+) derived from adult periodontal ligament. Shh components were localized to primary cilia in STRO-1(+) cells after Shh stimulation. STRO-1(+) also expressed Shh, suggesting an autocrine-regulated phenomenon. Thus, we propose that Shh plays a critical role in the regulation of cell proliferation in STRO-1(+)/HPLSC.",

keywords = "human periodontal ligament stem cells, primary cilia, proliferation, Sonic hedgehog",

author = "C. Mart{\'i}nez and Smith, {P. C.} and Rodriguez, {J. P.} and V. Palma",

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AU - Martínez, C.

AU - Smith, P. C.

AU - Rodriguez, J. P.

AU - Palma, V.

PY - 2011/4

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N2 - Regulation of cell renewal in the periodontium is a critical cell function that has not been clarified. Sonic hedgehog (Shh) is a secreted signaling molecule that plays a key role during development and adult tissue homeostasis. In the present study, we have analyzed the role played by Shh in human periodontal ligament stem cell (HPLSC) proliferation. HPLSC were isolated with anti-STRO-1 antibodies. Shh increased the expression of GLI1 and PTC-1 and selectively stimulated cell proliferation in STRO-1(+) derived from adult periodontal ligament. Shh components were localized to primary cilia in STRO-1(+) cells after Shh stimulation. STRO-1(+) also expressed Shh, suggesting an autocrine-regulated phenomenon. Thus, we propose that Shh plays a critical role in the regulation of cell proliferation in STRO-1(+)/HPLSC.

AB - Regulation of cell renewal in the periodontium is a critical cell function that has not been clarified. Sonic hedgehog (Shh) is a secreted signaling molecule that plays a key role during development and adult tissue homeostasis. In the present study, we have analyzed the role played by Shh in human periodontal ligament stem cell (HPLSC) proliferation. HPLSC were isolated with anti-STRO-1 antibodies. Shh increased the expression of GLI1 and PTC-1 and selectively stimulated cell proliferation in STRO-1(+) derived from adult periodontal ligament. Shh components were localized to primary cilia in STRO-1(+) cells after Shh stimulation. STRO-1(+) also expressed Shh, suggesting an autocrine-regulated phenomenon. Thus, we propose that Shh plays a critical role in the regulation of cell proliferation in STRO-1(+)/HPLSC.

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KW - primary cilia

KW - proliferation

KW - Sonic hedgehog

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Sonic hedgehog stimulates proliferation of human periodontal ligament stem cells

Abstract

Bibliographical note

Keywords

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