Sepsis progression to multiple organ dysfunction in carotid chemo/baro-denervated rats treated with lipopolysaccharide

Gino Nardocci; Aldo Martin; Sebastián Abarzúa; Jorge Rodríguez; Felipe Simon; Edison P. Reyes; Claudio Acuña-Castillo; Cristina Navarro; Paula P. Cortes; Ricardo Fernández

doi:10.1016/j.jneuroim.2014.12.002

Sepsis progression to multiple organ dysfunction in carotid chemo/baro-denervated rats treated with lipopolysaccharide

Gino Nardocci, Aldo Martin, Sebastián Abarzúa, Jorge Rodríguez, Felipe Simon, Edison P. Reyes, Claudio Acuña-Castillo, Cristina Navarro, Paula P. Cortes, Ricardo Fernández^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Sepsis progresses to multiple organ dysfunction (MOD) due to the uncontrolled release of inflammatory mediators. Carotid chemo/baro-receptors could play a protective role during sepsis. In anesthetized male rats, we measured cardiorespiratory variables and plasma TNF-α, glucocorticoids, epinephrine, and MOD marker levels 90. min after lipopolysaccharide (LPS) administration in control (SHAM surgery) and bilateral carotid chemo/baro-denervated (BCN) rats. BCN prior to LPS blunted the tachypneic response and enhanced tachycardia and hypotension. BCN-LPS rats also showed blunted plasma glucocorticoid responses, boosted epinephrine and TNF-α responses, and earlier MOD onset with a lower survival time compared with SHAM-LPS rats. Consequently, the complete absence of carotid chemo/baro-sensory function modified the neural, endocrine and inflammatory responses to sepsis. Thus, carotid chemo/baro-receptors play a protective role in sepsis.

Original language	English
Pages (from-to)	44-52
Number of pages	9
Journal	Journal of Neuroimmunology
Volume	278
DOIs	https://doi.org/10.1016/j.jneuroim.2014.12.002
State	Published - 15 Jan 2015
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by research grants from the Fondo Nacional de Desarrollo Científico y Tecnológico [FONDECYT 1120976 (RF), 1110734 (CAC), 3140414 (GN) and 1121078 (FS)], Dirección General de Investigación, Universidad Andrés Bello [UNAB DI-354-13/R (RF)], and the Millenium Institute on Immunology and Immunotherapy [ P09-016-F (FS)]. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Publisher Copyright:
© 2014 Elsevier B.V.

Keywords

Bilateral carotid/sinus neurotomy
Carotid body
Epinephrine
Glucocorticoids
Multiple organ dysfunction
Sepsis
TNF-α
Tissue damage

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10.1016/j.jneuroim.2014.12.002

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title = "Sepsis progression to multiple organ dysfunction in carotid chemo/baro-denervated rats treated with lipopolysaccharide",

abstract = "Sepsis progresses to multiple organ dysfunction (MOD) due to the uncontrolled release of inflammatory mediators. Carotid chemo/baro-receptors could play a protective role during sepsis. In anesthetized male rats, we measured cardiorespiratory variables and plasma TNF-α, glucocorticoids, epinephrine, and MOD marker levels 90. min after lipopolysaccharide (LPS) administration in control (SHAM surgery) and bilateral carotid chemo/baro-denervated (BCN) rats. BCN prior to LPS blunted the tachypneic response and enhanced tachycardia and hypotension. BCN-LPS rats also showed blunted plasma glucocorticoid responses, boosted epinephrine and TNF-α responses, and earlier MOD onset with a lower survival time compared with SHAM-LPS rats. Consequently, the complete absence of carotid chemo/baro-sensory function modified the neural, endocrine and inflammatory responses to sepsis. Thus, carotid chemo/baro-receptors play a protective role in sepsis.",

keywords = "Bilateral carotid/sinus neurotomy, Carotid body, Epinephrine, Glucocorticoids, Multiple organ dysfunction, Sepsis, TNF-α, Tissue damage",

author = "Gino Nardocci and Aldo Martin and Sebasti{\'a}n Abarz{\'u}a and Jorge Rodr{\'i}guez and Felipe Simon and Reyes, {Edison P.} and Claudio Acu{\~n}a-Castillo and Cristina Navarro and Cortes, {Paula P.} and Ricardo Fern{\'a}ndez",

note = "Funding Information: This work was supported by research grants from the Fondo Nacional de Desarrollo Cient{\'i}fico y Tecnol{\'o}gico [FONDECYT 1120976 (RF), 1110734 (CAC), 3140414 (GN) and 1121078 (FS)], Direcci{\'o}n General de Investigaci{\'o}n, Universidad Andr{\'e}s Bello [UNAB DI-354-13/R (RF)], and the Millenium Institute on Immunology and Immunotherapy [ P09-016-F (FS)]. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2014 Elsevier B.V.",

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AU - Nardocci, Gino

AU - Martin, Aldo

AU - Abarzúa, Sebastián

AU - Rodríguez, Jorge

AU - Simon, Felipe

AU - Reyes, Edison P.

AU - Acuña-Castillo, Claudio

AU - Navarro, Cristina

AU - Cortes, Paula P.

AU - Fernández, Ricardo

N1 - Funding Information: This work was supported by research grants from the Fondo Nacional de Desarrollo Científico y Tecnológico [FONDECYT 1120976 (RF), 1110734 (CAC), 3140414 (GN) and 1121078 (FS)], Dirección General de Investigación, Universidad Andrés Bello [UNAB DI-354-13/R (RF)], and the Millenium Institute on Immunology and Immunotherapy [ P09-016-F (FS)]. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: © 2014 Elsevier B.V.

PY - 2015/1/15

Y1 - 2015/1/15

N2 - Sepsis progresses to multiple organ dysfunction (MOD) due to the uncontrolled release of inflammatory mediators. Carotid chemo/baro-receptors could play a protective role during sepsis. In anesthetized male rats, we measured cardiorespiratory variables and plasma TNF-α, glucocorticoids, epinephrine, and MOD marker levels 90. min after lipopolysaccharide (LPS) administration in control (SHAM surgery) and bilateral carotid chemo/baro-denervated (BCN) rats. BCN prior to LPS blunted the tachypneic response and enhanced tachycardia and hypotension. BCN-LPS rats also showed blunted plasma glucocorticoid responses, boosted epinephrine and TNF-α responses, and earlier MOD onset with a lower survival time compared with SHAM-LPS rats. Consequently, the complete absence of carotid chemo/baro-sensory function modified the neural, endocrine and inflammatory responses to sepsis. Thus, carotid chemo/baro-receptors play a protective role in sepsis.

AB - Sepsis progresses to multiple organ dysfunction (MOD) due to the uncontrolled release of inflammatory mediators. Carotid chemo/baro-receptors could play a protective role during sepsis. In anesthetized male rats, we measured cardiorespiratory variables and plasma TNF-α, glucocorticoids, epinephrine, and MOD marker levels 90. min after lipopolysaccharide (LPS) administration in control (SHAM surgery) and bilateral carotid chemo/baro-denervated (BCN) rats. BCN prior to LPS blunted the tachypneic response and enhanced tachycardia and hypotension. BCN-LPS rats also showed blunted plasma glucocorticoid responses, boosted epinephrine and TNF-α responses, and earlier MOD onset with a lower survival time compared with SHAM-LPS rats. Consequently, the complete absence of carotid chemo/baro-sensory function modified the neural, endocrine and inflammatory responses to sepsis. Thus, carotid chemo/baro-receptors play a protective role in sepsis.

KW - Bilateral carotid/sinus neurotomy

KW - Carotid body

KW - Epinephrine

KW - Glucocorticoids

KW - Multiple organ dysfunction

KW - Sepsis

KW - TNF-α

KW - Tissue damage

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SN - 0165-5728

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JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

ER -

Sepsis progression to multiple organ dysfunction in carotid chemo/baro-denervated rats treated with lipopolysaccharide

Abstract

Bibliographical note

Keywords

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