Retinaldehyde dehydrogenase activity is triggered during allograft rejection and it drives Th1/Th17 cytokine production

Rodrigo A. Morales, Mauricio Campos-Mora, Tania Gajardo, Francisco Pérez, Javier Campos, Juan C. Aguillón, Karina Pino-Lagos

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Retinoic acid (RA), a vitamin A metabolite, has been attributed to relevant functions in adaptive immunity. On T cells, the disruption on RA signaling alters both CD4+ and CD8+ T cells effector function. In this study, we evaluated the contribution of RA synthesis during the immune response using an in vivo skin transplantation model. Our data indicates that the frequency and number of cells containing an active retinaldehyde dehydrogenase (RALDH), a key enzyme for RA synthesis, is increased during skin transplant rejection. In addition, we found that the expression of the mRNA coding for the isoform RALDH2 is up-regulated on graft rejecting draining lymph nodes (dLNs) cells. Lastly, we observed that IFN-γ and IL-17 production by ex vivo re-stimulated dLNs cells is greatly increased during rejection, which it turns depends on RA synthesis, as shown in experiments using a specific RALDH inhibitor. Altogether, our data demonstrate that RA synthesis is incremented during the immune response against an allograft, and also indicates that the synthesis of RA is required for cytokine production by dLNs resident T cells.

Original languageEnglish
Pages (from-to)769-774
Number of pages6
JournalImmunobiology
Volume220
Issue number6
DOIs
StatePublished - 1 Jun 2015
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by FONDECYT Grant 11121309 .

Publisher Copyright:
© 2014 Elsevier GmbH.

Keywords

  • Rejection
  • Retinoic acid
  • Th1/Th17
  • Transplantation

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