Regenerating axons of the rat require a local source of proteins

Jorge Eduardo Gaete Olivares, Gonzalo Kameid, Jaime Alvarez

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Regenerating axons need proteins to grow and we explored whether a local supply is necessary. Crushed peroneal nerves were entubulated with silicone sleeves, plain or loaded with cycloheximide (CHX); some nerves were frozen to kill resident cells. When a plain sleeve was placed distal to the crush, axons regrew 5.0 mm in 3 days (pinch test), and 4.6 mm when the sleeve was placed around a frozen nerve (n.s). CHX administered distal to the crush reduced the elongation by ~58% (P < 0.01) in unfrozen or frozen nerves whilst its administration central to the crush was ineffectual. Immunostaining of nerves with GAP-43 gave similar values. Under the electron microscope, axonal sprouts were less frequent when CHX was used irrespective of the cellular or acellular condition of the nerve. Therefore, an inhibitor of protein synthesis reduces axonal regrowth, an effect mediated neither by parent neurones nor by resident cells. We propose that axons synthesize proteins.

Original languageEnglish
Pages (from-to)197-200
Number of pages4
JournalNeuroscience Letters
Volume251
Issue number3
DOIs
StatePublished - 31 Jul 1998
Externally publishedYes

Bibliographical note

Funding Information:
We thank L. Villarroel for statistical assistance, and G. Méndez, J. Morillas, and M. Pérez for technical assistance. This work was supported by grants FONDECYT 1980973, and Deutsche Gesellschaft für Technische Zusammenarbeit GmbH.

Keywords

  • Cycloheximide
  • Nerve fibre
  • Protein synthesis
  • Schwann cell
  • Slow transport

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