Regenerating axons of the rat require a local source of proteins

Jorge Gaete, Gonzalo Kameid, Jaime Alvarez

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33 Scopus citations


Regenerating axons need proteins to grow and we explored whether a local supply is necessary. Crushed peroneal nerves were entubulated with silicone sleeves, plain or loaded with cycloheximide (CHX); some nerves were frozen to kill resident cells. When a plain sleeve was placed distal to the crush, axons regrew 5.0 mm in 3 days (pinch test), and 4.6 mm when the sleeve was placed around a frozen nerve (n.s). CHX administered distal to the crush reduced the elongation by ~58% (P < 0.01) in unfrozen or frozen nerves whilst its administration central to the crush was ineffectual. Immunostaining of nerves with GAP-43 gave similar values. Under the electron microscope, axonal sprouts were less frequent when CHX was used irrespective of the cellular or acellular condition of the nerve. Therefore, an inhibitor of protein synthesis reduces axonal regrowth, an effect mediated neither by parent neurones nor by resident cells. We propose that axons synthesize proteins.
Original languageAmerican English
Pages (from-to)197-200
Number of pages4
JournalNeuroscience Letters
Issue number3
StatePublished - 31 Jul 1998
Externally publishedYes


  • Cycloheximide
  • Nerve fibre
  • Protein synthesis
  • Schwann cell
  • Slow transport

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