Rapid fabrication of reinforced and cell-laden vascular grafts structurally inspired by human coronary arteries

Tamara L. Akentjew; Claudia Terraza; Cristian Suazo; Jekaterina Maksimuck; Camila A. Wilkens; Francisco Vargas; Gabriela Zavala; Macarena Ocaña; Javier Enrione; Claudio M. García-Herrera; Loreto M. Valenzuela; Jonny J. Blaker; Maroun Khoury; Juan Pablo Acevedo

doi:10.1038/s41467-019-11090-3

Rapid fabrication of reinforced and cell-laden vascular grafts structurally inspired by human coronary arteries

Tamara L. Akentjew, Claudia Terraza, Cristian Suazo, Jekaterina Maksimuck, Camila A. Wilkens, Francisco Vargas, Gabriela Zavala, Macarena Ocaña, Javier Enrione, Claudio M. García-Herrera, Loreto M. Valenzuela, Jonny J. Blaker, Maroun Khoury, Juan Pablo Acevedo^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

Design strategies for small diameter vascular grafts are converging toward native-inspired tissue engineered grafts. A new automated technology is presented that combines a dip-spinning methodology for depositioning concentric cell-laden hydrogel layers, with an adapted solution blow spinning (SBS) device for intercalated placement of aligned reinforcement nanofibres. This additive manufacture approach allows the assembly of bio-inspired structural configurations of concentric cell patterns with fibres at specific angles and wavy arrangements. The middle and outer layers were tuned to structurally mimic the media and adventitia layers of native arteries, enabling the fabrication of small bore grafts that exhibit the J-shape mechanical response and compliance of human coronary arteries. This scalable automated system can fabricate cellularized multilayer grafts within 30 min. Grafts were evaluated by hemocompatibility studies and a preliminary in vivo carotid rabbit model. The dip-spinning-SBS technology generates constructs with native mechanical properties and cell-derived biological activities, critical for clinical bypass applications.

Original language	English
Article number	3098
Journal	Nature Communications
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-11090-3
State	Published - 1 Dec 2019

Bibliographical note

Funding Information:
We want to thank all the researchers in the Cells for Cells, Dr. Valenzuela (Pontificia Universidad Católica de Chile), the Bio-Active Materials group (The University of Manchester, UK) and Dr. García’s (Universidad de Santiago de Chile) laboratories for insightful discussions and suggestions. We thank Dr. Gowsihan Poologasundarampillai for assistance with synchrotron-CT and the Diamond Light Source for beamtime (MT15507) on the I13 Diamond-Manchester Branchline and the staff there for all of the assistance with data collection. This work was made possible by the facilities and support provided by the Diamond-Manchester Collaboration and the Research Complex at Harwell, funded in part by the Engineering and Physical Sciences Research Council (EPSRC) (EP/M023877/1). We acknowledge financial support from CORFO 18COTE-97983, FONDEF IDEA I15I10545, CONICYT by the grants FONDECYT Nos 3160680, 1170608, FONDEQUIP EQM130028 and Newton-Picarte REDES No. 140144 and the Universidad de los Andes PMI program UAN1301.

Publisher Copyright:
© 2019, The Author(s).

Keywords

Bioprosthesis
Blood Vessel Prosthesis
Blood Vessel Prosthesis Implantation
Coronary Artery Bypass
Coronary Vessels
Human Umbilical Vein Endothelial Cells
Humans
Hydrogels
Materials Testing
Tensile Strength
Tissue Engineering

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10.1038/s41467-019-11090-3

Cite this

Akentjew, T. L., Terraza, C., Suazo, C., Maksimuck, J., Wilkens, C. A., Vargas, F., Zavala, G., Ocaña, M., Enrione, J., García-Herrera, C. M., Valenzuela, L. M., Blaker, J. J., Khoury, M., & Acevedo, J. P. (2019). Rapid fabrication of reinforced and cell-laden vascular grafts structurally inspired by human coronary arteries. Nature Communications, 10(1), Article 3098. https://doi.org/10.1038/s41467-019-11090-3

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title = "Rapid fabrication of reinforced and cell-laden vascular grafts structurally inspired by human coronary arteries",

abstract = "Design strategies for small diameter vascular grafts are converging toward native-inspired tissue engineered grafts. A new automated technology is presented that combines a dip-spinning methodology for depositioning concentric cell-laden hydrogel layers, with an adapted solution blow spinning (SBS) device for intercalated placement of aligned reinforcement nanofibres. This additive manufacture approach allows the assembly of bio-inspired structural configurations of concentric cell patterns with fibres at specific angles and wavy arrangements. The middle and outer layers were tuned to structurally mimic the media and adventitia layers of native arteries, enabling the fabrication of small bore grafts that exhibit the J-shape mechanical response and compliance of human coronary arteries. This scalable automated system can fabricate cellularized multilayer grafts within 30 min. Grafts were evaluated by hemocompatibility studies and a preliminary in vivo carotid rabbit model. The dip-spinning-SBS technology generates constructs with native mechanical properties and cell-derived biological activities, critical for clinical bypass applications.",

keywords = "Bioprosthesis, Blood Vessel Prosthesis, Blood Vessel Prosthesis Implantation, Coronary Artery Bypass, Coronary Vessels, Human Umbilical Vein Endothelial Cells, Humans, Hydrogels, Materials Testing, Tensile Strength, Tissue Engineering",

author = "Akentjew, {Tamara L.} and Claudia Terraza and Cristian Suazo and Jekaterina Maksimuck and Wilkens, {Camila A.} and Francisco Vargas and Gabriela Zavala and Macarena Oca{\~n}a and Javier Enrione and Garc{\'i}a-Herrera, {Claudio M.} and Valenzuela, {Loreto M.} and Blaker, {Jonny J.} and Maroun Khoury and Acevedo, {Juan Pablo}",

note = "Funding Information: We want to thank all the researchers in the Cells for Cells, Dr. Valenzuela (Pontificia Universidad Cat{\'o}lica de Chile), the Bio-Active Materials group (The University of Manchester, UK) and Dr. Garc{\'i}a{\textquoteright}s (Universidad de Santiago de Chile) laboratories for insightful discussions and suggestions. We thank Dr. Gowsihan Poologasundarampillai for assistance with synchrotron-CT and the Diamond Light Source for beamtime (MT15507) on the I13 Diamond-Manchester Branchline and the staff there for all of the assistance with data collection. This work was made possible by the facilities and support provided by the Diamond-Manchester Collaboration and the Research Complex at Harwell, funded in part by the Engineering and Physical Sciences Research Council (EPSRC) (EP/M023877/1). We acknowledge financial support from CORFO 18COTE-97983, FONDEF IDEA I15I10545, CONICYT by the grants FONDECYT Nos 3160680, 1170608, FONDEQUIP EQM130028 and Newton-Picarte REDES No. 140144 and the Universidad de los Andes PMI program UAN1301. Publisher Copyright: {\textcopyright} 2019, The Author(s).",

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doi = "10.1038/s41467-019-11090-3",

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Akentjew, TL, Terraza, C, Suazo, C, Maksimuck, J, Wilkens, CA, Vargas, F, Zavala, G, Ocaña, M, Enrione, J, García-Herrera, CM, Valenzuela, LM, Blaker, JJ, Khoury, M & Acevedo, JP 2019, 'Rapid fabrication of reinforced and cell-laden vascular grafts structurally inspired by human coronary arteries', Nature Communications, vol. 10, no. 1, 3098. https://doi.org/10.1038/s41467-019-11090-3

TY - JOUR

T1 - Rapid fabrication of reinforced and cell-laden vascular grafts structurally inspired by human coronary arteries

AU - Akentjew, Tamara L.

AU - Terraza, Claudia

AU - Suazo, Cristian

AU - Maksimuck, Jekaterina

AU - Wilkens, Camila A.

AU - Vargas, Francisco

AU - Zavala, Gabriela

AU - Ocaña, Macarena

AU - Enrione, Javier

AU - García-Herrera, Claudio M.

AU - Valenzuela, Loreto M.

AU - Blaker, Jonny J.

AU - Khoury, Maroun

AU - Acevedo, Juan Pablo

N1 - Funding Information: We want to thank all the researchers in the Cells for Cells, Dr. Valenzuela (Pontificia Universidad Católica de Chile), the Bio-Active Materials group (The University of Manchester, UK) and Dr. García’s (Universidad de Santiago de Chile) laboratories for insightful discussions and suggestions. We thank Dr. Gowsihan Poologasundarampillai for assistance with synchrotron-CT and the Diamond Light Source for beamtime (MT15507) on the I13 Diamond-Manchester Branchline and the staff there for all of the assistance with data collection. This work was made possible by the facilities and support provided by the Diamond-Manchester Collaboration and the Research Complex at Harwell, funded in part by the Engineering and Physical Sciences Research Council (EPSRC) (EP/M023877/1). We acknowledge financial support from CORFO 18COTE-97983, FONDEF IDEA I15I10545, CONICYT by the grants FONDECYT Nos 3160680, 1170608, FONDEQUIP EQM130028 and Newton-Picarte REDES No. 140144 and the Universidad de los Andes PMI program UAN1301. Publisher Copyright: © 2019, The Author(s).

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Design strategies for small diameter vascular grafts are converging toward native-inspired tissue engineered grafts. A new automated technology is presented that combines a dip-spinning methodology for depositioning concentric cell-laden hydrogel layers, with an adapted solution blow spinning (SBS) device for intercalated placement of aligned reinforcement nanofibres. This additive manufacture approach allows the assembly of bio-inspired structural configurations of concentric cell patterns with fibres at specific angles and wavy arrangements. The middle and outer layers were tuned to structurally mimic the media and adventitia layers of native arteries, enabling the fabrication of small bore grafts that exhibit the J-shape mechanical response and compliance of human coronary arteries. This scalable automated system can fabricate cellularized multilayer grafts within 30 min. Grafts were evaluated by hemocompatibility studies and a preliminary in vivo carotid rabbit model. The dip-spinning-SBS technology generates constructs with native mechanical properties and cell-derived biological activities, critical for clinical bypass applications.

AB - Design strategies for small diameter vascular grafts are converging toward native-inspired tissue engineered grafts. A new automated technology is presented that combines a dip-spinning methodology for depositioning concentric cell-laden hydrogel layers, with an adapted solution blow spinning (SBS) device for intercalated placement of aligned reinforcement nanofibres. This additive manufacture approach allows the assembly of bio-inspired structural configurations of concentric cell patterns with fibres at specific angles and wavy arrangements. The middle and outer layers were tuned to structurally mimic the media and adventitia layers of native arteries, enabling the fabrication of small bore grafts that exhibit the J-shape mechanical response and compliance of human coronary arteries. This scalable automated system can fabricate cellularized multilayer grafts within 30 min. Grafts were evaluated by hemocompatibility studies and a preliminary in vivo carotid rabbit model. The dip-spinning-SBS technology generates constructs with native mechanical properties and cell-derived biological activities, critical for clinical bypass applications.

KW - Bioprosthesis

KW - Blood Vessel Prosthesis

KW - Blood Vessel Prosthesis Implantation

KW - Coronary Artery Bypass

KW - Coronary Vessels

KW - Human Umbilical Vein Endothelial Cells

KW - Humans

KW - Hydrogels

KW - Materials Testing

KW - Tensile Strength

KW - Tissue Engineering

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DO - 10.1038/s41467-019-11090-3

M3 - Article

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SN - 2041-1723

VL - 10

JO - Nature Communications

JF - Nature Communications

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M1 - 3098

ER -

Rapid fabrication of reinforced and cell-laden vascular grafts structurally inspired by human coronary arteries

Abstract

Bibliographical note

Keywords

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