Rapid fabrication of reinforced and cell-laden vascular grafts structurally inspired by human coronary arteries

Tamara L. Akentjew, Claudia Terraza, Cristian Suazo, Jekaterina Maksimuck, Camila A. Wilkens, Francisco Vargas, Gabriela Zavala, Macarena Ocaña, Javier Enrione, Claudio M. García-Herrera, Loreto M. Valenzuela, Jonny J. Blaker, Maroun Khoury, Juan Pablo Acevedo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

52 Scopus citations


Design strategies for small diameter vascular grafts are converging toward native-inspired tissue engineered grafts. A new automated technology is presented that combines a dip-spinning methodology for depositioning concentric cell-laden hydrogel layers, with an adapted solution blow spinning (SBS) device for intercalated placement of aligned reinforcement nanofibres. This additive manufacture approach allows the assembly of bio-inspired structural configurations of concentric cell patterns with fibres at specific angles and wavy arrangements. The middle and outer layers were tuned to structurally mimic the media and adventitia layers of native arteries, enabling the fabrication of small bore grafts that exhibit the J-shape mechanical response and compliance of human coronary arteries. This scalable automated system can fabricate cellularized multilayer grafts within 30 min. Grafts were evaluated by hemocompatibility studies and a preliminary in vivo carotid rabbit model. The dip-spinning-SBS technology generates constructs with native mechanical properties and cell-derived biological activities, critical for clinical bypass applications.

Original languageEnglish
Article number3098
JournalNature Communications
Issue number1
StatePublished - 1 Dec 2019

Bibliographical note

Funding Information:
We want to thank all the researchers in the Cells for Cells, Dr. Valenzuela (Pontificia Universidad Católica de Chile), the Bio-Active Materials group (The University of Manchester, UK) and Dr. García’s (Universidad de Santiago de Chile) laboratories for insightful discussions and suggestions. We thank Dr. Gowsihan Poologasundarampillai for assistance with synchrotron-CT and the Diamond Light Source for beamtime (MT15507) on the I13 Diamond-Manchester Branchline and the staff there for all of the assistance with data collection. This work was made possible by the facilities and support provided by the Diamond-Manchester Collaboration and the Research Complex at Harwell, funded in part by the Engineering and Physical Sciences Research Council (EPSRC) (EP/M023877/1). We acknowledge financial support from CORFO 18COTE-97983, FONDEF IDEA I15I10545, CONICYT by the grants FONDECYT Nos 3160680, 1170608, FONDEQUIP EQM130028 and Newton-Picarte REDES No. 140144 and the Universidad de los Andes PMI program UAN1301.

Publisher Copyright:
© 2019, The Author(s).


  • Bioprosthesis
  • Blood Vessel Prosthesis
  • Blood Vessel Prosthesis Implantation
  • Coronary Artery Bypass
  • Coronary Vessels
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Hydrogels
  • Materials Testing
  • Tensile Strength
  • Tissue Engineering


Dive into the research topics of 'Rapid fabrication of reinforced and cell-laden vascular grafts structurally inspired by human coronary arteries'. Together they form a unique fingerprint.

Cite this