Pyrroline-5-Carboxylate Reductase 1 Directs the Cartilage Protective and Regenerative Potential of Murphy Roths Large Mouse Mesenchymal Stem Cells

Gautier Tejedor; Rafael Contreras-Lopez; Audrey Barthelaix; Maxime Ruiz; Danièle Noël; Frédéric De Ceuninck; Philippe Pastoureau; Patricia Alejandra Luz Crawford; Christian Jorgensen; Farida Djouad

doi:10.3389/fcell.2021.604756

Pyrroline-5-Carboxylate Reductase 1 Directs the Cartilage Protective and Regenerative Potential of Murphy Roths Large Mouse Mesenchymal Stem Cells

Gautier Tejedor, Rafael Contreras-Lopez, Audrey Barthelaix, Maxime Ruiz, Danièle Noël, Frédéric De Ceuninck, Philippe Pastoureau, Patricia Alejandra Luz Crawford, Christian Jorgensen, Farida Djouad^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Murphy Roths Large (MRL) mice possess outstanding capacity to regenerate several tissues. In the present study, we investigated whether this regenerative potential could be associated with the intrinsic particularities possessed by their mesenchymal stem cells (MSCs). We demonstrated that MSCs derived from MRL mice (MRL MSCs) display a superior chondrogenic potential than do C57BL/6 MSC (BL6 MSCs). This higher chondrogenic potential of MRL MSCs was associated with a higher expression level of pyrroline-5-carboxylate reductase 1 (PYCR1), an enzyme that catalyzes the biosynthesis of proline, in MRL MSCs compared with BL6 MSCs. The knockdown of PYCR1 in MRL MSCs, using a specific small interfering RNA (siRNA), abolishes their chondrogenic potential. Moreover, we showed that PYCR1 silencing in MRL MSCs induced a metabolic switch from glycolysis to oxidative phosphorylation. In two in vitro chondrocyte models that reproduce the main features of osteoarthritis (OA) chondrocytes including a downregulation of chondrocyte markers, a significant decrease of PYCR1 was observed. A downregulation of chondrocyte markers was also observed by silencing PYCR1 in freshly isolated healthy chondrocytes. Regarding MSC chondroprotective properties on chondrocytes with OA features, we showed that MSCs silenced for PYCR1 failed to protect chondrocytes from a reduced expression of anabolic markers, while MSCs overexpressing PYCR1 exhibited an increased chondroprotective potential. Finally, using the ear punch model, we demonstrated that MRL MSCs induced a regenerative response in non-regenerating BL6 mice, while BL6 and MRL MSCs deficient for PYCR1 did not. In conclusion, our results provide evidence that MRL mouse regenerative potential is, in part, attributed to its MSCs that exhibit higher PYCR1-dependent glycolytic potential, differentiation capacities, chondroprotective abilities, and regenerative potential than BL6 MSCs.

Original language	English
Article number	604756
Pages (from-to)	604756
Journal	Frontiers in Cell and Developmental Biology
Volume	9
DOIs	https://doi.org/10.3389/fcell.2021.604756
State	Published - 2 Jul 2021

Bibliographical note

Funding Information:
This work was supported by INSERM, the University of Montpellier. We thank Servier for financial support of this project.

Funding Information:
The authors declare that this study received funding from Servier. The funder was not involved in the study design, collection, analysis, interpretation of data and the writing of this article for publication.

Publisher Copyright:
© Copyright © 2021 Tejedor, Contreras-Lopez, Barthelaix, Ruiz, Noël, De Ceuninck, Pastoureau, Luz-Crawford, Jorgensen and Djouad.

Copyright © 2021 Tejedor, Contreras-Lopez, Barthelaix, Ruiz, Noël, De Ceuninck, Pastoureau, Luz-Crawford, Jorgensen and Djouad.

Keywords

chondrocyte
chondrogenesis
chondroprotection
mesenchymal stem cells
metabolism
MRL mouse
PYCR1
regeneration

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10.3389/fcell.2021.604756

Cite this

Tejedor, G., Contreras-Lopez, R., Barthelaix, A., Ruiz, M., Noël, D., De Ceuninck, F., Pastoureau, P., Luz Crawford, P. A., Jorgensen, C., & Djouad, F. (2021). Pyrroline-5-Carboxylate Reductase 1 Directs the Cartilage Protective and Regenerative Potential of Murphy Roths Large Mouse Mesenchymal Stem Cells. Frontiers in Cell and Developmental Biology, 9, 604756. Article 604756. https://doi.org/10.3389/fcell.2021.604756

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title = "Pyrroline-5-Carboxylate Reductase 1 Directs the Cartilage Protective and Regenerative Potential of Murphy Roths Large Mouse Mesenchymal Stem Cells",

abstract = "Murphy Roths Large (MRL) mice possess outstanding capacity to regenerate several tissues. In the present study, we investigated whether this regenerative potential could be associated with the intrinsic particularities possessed by their mesenchymal stem cells (MSCs). We demonstrated that MSCs derived from MRL mice (MRL MSCs) display a superior chondrogenic potential than do C57BL/6 MSC (BL6 MSCs). This higher chondrogenic potential of MRL MSCs was associated with a higher expression level of pyrroline-5-carboxylate reductase 1 (PYCR1), an enzyme that catalyzes the biosynthesis of proline, in MRL MSCs compared with BL6 MSCs. The knockdown of PYCR1 in MRL MSCs, using a specific small interfering RNA (siRNA), abolishes their chondrogenic potential. Moreover, we showed that PYCR1 silencing in MRL MSCs induced a metabolic switch from glycolysis to oxidative phosphorylation. In two in vitro chondrocyte models that reproduce the main features of osteoarthritis (OA) chondrocytes including a downregulation of chondrocyte markers, a significant decrease of PYCR1 was observed. A downregulation of chondrocyte markers was also observed by silencing PYCR1 in freshly isolated healthy chondrocytes. Regarding MSC chondroprotective properties on chondrocytes with OA features, we showed that MSCs silenced for PYCR1 failed to protect chondrocytes from a reduced expression of anabolic markers, while MSCs overexpressing PYCR1 exhibited an increased chondroprotective potential. Finally, using the ear punch model, we demonstrated that MRL MSCs induced a regenerative response in non-regenerating BL6 mice, while BL6 and MRL MSCs deficient for PYCR1 did not. In conclusion, our results provide evidence that MRL mouse regenerative potential is, in part, attributed to its MSCs that exhibit higher PYCR1-dependent glycolytic potential, differentiation capacities, chondroprotective abilities, and regenerative potential than BL6 MSCs.",

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author = "Gautier Tejedor and Rafael Contreras-Lopez and Audrey Barthelaix and Maxime Ruiz and Dani{\`e}le No{\"e}l and {De Ceuninck}, Fr{\'e}d{\'e}ric and Philippe Pastoureau and {Luz Crawford}, {Patricia Alejandra} and Christian Jorgensen and Farida Djouad",

note = "Funding Information: This work was supported by INSERM, the University of Montpellier. We thank Servier for financial support of this project. Funding Information: The authors declare that this study received funding from Servier. The funder was not involved in the study design, collection, analysis, interpretation of data and the writing of this article for publication. Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Tejedor, Contreras-Lopez, Barthelaix, Ruiz, No{\"e}l, De Ceuninck, Pastoureau, Luz-Crawford, Jorgensen and Djouad. Copyright {\textcopyright} 2021 Tejedor, Contreras-Lopez, Barthelaix, Ruiz, No{\"e}l, De Ceuninck, Pastoureau, Luz-Crawford, Jorgensen and Djouad.",

year = "2021",

month = jul,

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doi = "10.3389/fcell.2021.604756",

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Tejedor, G, Contreras-Lopez, R, Barthelaix, A, Ruiz, M, Noël, D, De Ceuninck, F, Pastoureau, P, Luz Crawford, PA, Jorgensen, C & Djouad, F 2021, 'Pyrroline-5-Carboxylate Reductase 1 Directs the Cartilage Protective and Regenerative Potential of Murphy Roths Large Mouse Mesenchymal Stem Cells', Frontiers in Cell and Developmental Biology, vol. 9, 604756, pp. 604756. https://doi.org/10.3389/fcell.2021.604756

Pyrroline-5-Carboxylate Reductase 1 Directs the Cartilage Protective and Regenerative Potential of Murphy Roths Large Mouse Mesenchymal Stem Cells. / Tejedor, Gautier; Contreras-Lopez, Rafael; Barthelaix, Audrey et al.
In: Frontiers in Cell and Developmental Biology, Vol. 9, 604756, 02.07.2021, p. 604756.

Research output: Contribution to journal › Article › peer-review

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AU - Tejedor, Gautier

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AU - Ruiz, Maxime

AU - Noël, Danièle

AU - De Ceuninck, Frédéric

AU - Pastoureau, Philippe

AU - Luz Crawford, Patricia Alejandra

AU - Jorgensen, Christian

AU - Djouad, Farida

N1 - Funding Information: This work was supported by INSERM, the University of Montpellier. We thank Servier for financial support of this project. Funding Information: The authors declare that this study received funding from Servier. The funder was not involved in the study design, collection, analysis, interpretation of data and the writing of this article for publication. Publisher Copyright: © Copyright © 2021 Tejedor, Contreras-Lopez, Barthelaix, Ruiz, Noël, De Ceuninck, Pastoureau, Luz-Crawford, Jorgensen and Djouad. Copyright © 2021 Tejedor, Contreras-Lopez, Barthelaix, Ruiz, Noël, De Ceuninck, Pastoureau, Luz-Crawford, Jorgensen and Djouad.

PY - 2021/7/2

Y1 - 2021/7/2

N2 - Murphy Roths Large (MRL) mice possess outstanding capacity to regenerate several tissues. In the present study, we investigated whether this regenerative potential could be associated with the intrinsic particularities possessed by their mesenchymal stem cells (MSCs). We demonstrated that MSCs derived from MRL mice (MRL MSCs) display a superior chondrogenic potential than do C57BL/6 MSC (BL6 MSCs). This higher chondrogenic potential of MRL MSCs was associated with a higher expression level of pyrroline-5-carboxylate reductase 1 (PYCR1), an enzyme that catalyzes the biosynthesis of proline, in MRL MSCs compared with BL6 MSCs. The knockdown of PYCR1 in MRL MSCs, using a specific small interfering RNA (siRNA), abolishes their chondrogenic potential. Moreover, we showed that PYCR1 silencing in MRL MSCs induced a metabolic switch from glycolysis to oxidative phosphorylation. In two in vitro chondrocyte models that reproduce the main features of osteoarthritis (OA) chondrocytes including a downregulation of chondrocyte markers, a significant decrease of PYCR1 was observed. A downregulation of chondrocyte markers was also observed by silencing PYCR1 in freshly isolated healthy chondrocytes. Regarding MSC chondroprotective properties on chondrocytes with OA features, we showed that MSCs silenced for PYCR1 failed to protect chondrocytes from a reduced expression of anabolic markers, while MSCs overexpressing PYCR1 exhibited an increased chondroprotective potential. Finally, using the ear punch model, we demonstrated that MRL MSCs induced a regenerative response in non-regenerating BL6 mice, while BL6 and MRL MSCs deficient for PYCR1 did not. In conclusion, our results provide evidence that MRL mouse regenerative potential is, in part, attributed to its MSCs that exhibit higher PYCR1-dependent glycolytic potential, differentiation capacities, chondroprotective abilities, and regenerative potential than BL6 MSCs.

AB - Murphy Roths Large (MRL) mice possess outstanding capacity to regenerate several tissues. In the present study, we investigated whether this regenerative potential could be associated with the intrinsic particularities possessed by their mesenchymal stem cells (MSCs). We demonstrated that MSCs derived from MRL mice (MRL MSCs) display a superior chondrogenic potential than do C57BL/6 MSC (BL6 MSCs). This higher chondrogenic potential of MRL MSCs was associated with a higher expression level of pyrroline-5-carboxylate reductase 1 (PYCR1), an enzyme that catalyzes the biosynthesis of proline, in MRL MSCs compared with BL6 MSCs. The knockdown of PYCR1 in MRL MSCs, using a specific small interfering RNA (siRNA), abolishes their chondrogenic potential. Moreover, we showed that PYCR1 silencing in MRL MSCs induced a metabolic switch from glycolysis to oxidative phosphorylation. In two in vitro chondrocyte models that reproduce the main features of osteoarthritis (OA) chondrocytes including a downregulation of chondrocyte markers, a significant decrease of PYCR1 was observed. A downregulation of chondrocyte markers was also observed by silencing PYCR1 in freshly isolated healthy chondrocytes. Regarding MSC chondroprotective properties on chondrocytes with OA features, we showed that MSCs silenced for PYCR1 failed to protect chondrocytes from a reduced expression of anabolic markers, while MSCs overexpressing PYCR1 exhibited an increased chondroprotective potential. Finally, using the ear punch model, we demonstrated that MRL MSCs induced a regenerative response in non-regenerating BL6 mice, while BL6 and MRL MSCs deficient for PYCR1 did not. In conclusion, our results provide evidence that MRL mouse regenerative potential is, in part, attributed to its MSCs that exhibit higher PYCR1-dependent glycolytic potential, differentiation capacities, chondroprotective abilities, and regenerative potential than BL6 MSCs.

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Pyrroline-5-Carboxylate Reductase 1 Directs the Cartilage Protective and Regenerative Potential of Murphy Roths Large Mouse Mesenchymal Stem Cells

Abstract

Bibliographical note

Keywords

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