The present work is focused on the formulation of core-shell nanoparticles via the layer-by-layer (L-b-L) self-assembly technique for delivery of biomacromolecules such as bone growth factors. The drug encapsulation efficiency of liposomes is enhanced with the increased stability of polyelectrolyte systems achieved through the alternate adsorption of several layers of natural polymers: anionic alginate and cationic chitosan on cationic nanosized phospholipid vesicles. The resulting particles were characterized for their size, surface charge, morphology, encapsulation efficiency, loading capacity and release kinetics over an extended period of 30 days. The L-b-L deposition technique succeeded in building a spherical, monodisperse and stable hybrid nanoparticulate protein delivery system with a cumulative size of 383 ± 11.5 nm and zeta potential surface charge of 44.61 ± 3.31 mV for five bilayered liposomes. The system offers numerous compartments for encapsulation including the aqueous core and within the polyelectrolyte shell demonstrating good entrapment and sustained linear release of a model protein, bovine serum albumin, in vitro. Our results demonstrate that this delivery system features an extended shelf life and can be loaded immediately prior to administration, thus preventing any loss of the protein.
|Number of pages
|Published - Mar 2008
Bibliographical noteFunding Information:
This work was supported by research grants from the Natural Sciences and Engineering Research Council (NSERC) and the Centre for Biorecognition and Biosensors (CBB). Dr. Ziyad Haidar acknowledges a post-graduate scholarship from the Canadian Institutes for Health Research (CIHR) Skeletal Health Training Program and Center for Bone and Periodontal Research in Montréal. The authors thank Dr. Julien Fatisson for his assistance in characterization, Mrs. L. Mongeon and Dr. L. Marcotte for their assistance in microscopy and Dr. F. Azari for her assistance in the protein loading and release study.
- Controlled drug release