Production of (R)-3-hydroxybutyric acid from methane by in vivo depolymerization of polyhydroxybutyrate in Methylocystis parvus OBBP

Luz Yáñez, Yadira Rodríguez, Felipe Scott, Alberto Vergara-Fernández, Raúl Muñoz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Methylocystis parvus OBBP accumulates polyhydroxybutyrate (PHB) using methane as the sole carbon and energy source. In this work, the feasibility of producing (R)-3-hydroxybutyric acid (R3HBA) via intracellularly accumulated PHB through depolymerization (in-vivo) was investigated. Results showed that a PHB to R3HBA conversion of 77.2 ± 0.9% (R3HBA titer of 0.153 ± 0.002 g L-1) can be attained in a mineral medium containing 1 g L-1 KNO3 at 30 °C with shaking at 200 rpm and a constant pH of 11 for 72 h. Nitrogen deprivation and neutral or acidic pHs strongly reduced the excreted R3HBA concentration. Reduced oxygen availability negatively affected the R3HBA yield, which decreased to 73.6 ± 4.9% (titer of 0.139 ± 0.01 g L-1) under microaerobic conditions. Likewise, the presence of increasing concentrations of R3HBA in the medium before the onset of PHB depolymerization reduced the initial R3HBA release rate and R3HBA yield.

Original languageEnglish
Article number127141
Pages (from-to)127141
JournalBioresource Technology
Volume353
DOIs
StatePublished - Jun 2022

Bibliographical note

Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.

Keywords

  • (R)-3-hydroxybutyrate
  • Chiral compounds
  • Greenhouse gas valorization
  • Methanotrophic bacteria
  • Polyhydroxybutyrate

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