Production of antihypertensive and antidiabetic peptide fractions from quinoa (Chenopodium quinoa Willd.) by electrodialysis with ultrafiltration membranes

Adrián González-Muñoz; Marion Valle; Rotimi E. Aluko; Laurent Bazinet; Javier Enrione

doi:10.1016/j.fshw.2022.06.024

Production of antihypertensive and antidiabetic peptide fractions from quinoa (Chenopodium quinoa Willd.) by electrodialysis with ultrafiltration membranes

Adrián González-Muñoz, Marion Valle, Rotimi E. Aluko, Laurent Bazinet^*, Javier Enrione^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Processing bioactive peptides from natural sources using electrodialysis with ultrafiltration membranes (EDUF) have gained attention since it can fractionate in terms of their charge and molecular weight. Quinoa is a pseudo-cereal highlighted by its high protein content, amino acid profile and adapting growing conditions. The present work aimed at the production of quinoa peptides through fractionation using EDUF and to test the fractions according to antihypertensive and antidiabetic activity. Experimental data showed the production of peptides ranging between 0.4 and 1.5 kDa. Cationic (CQPF) (3.01 %), anionic (AQPF) (1.18 %) and the electrically neutral fraction quinoa protein hydrolysate (QPH)-EDUF (∼95 %) were obtained. In-vitro studies showed the highest glucose uptake modulation in L6 cell skeletal myoblasts in presence of QPH-EDUF and AQPF (17 % and 11 %) indicating potential antidiabetic activity. The antihypertensive effect studied in-vivo in spontaneously hypertensive rats (SHR), showed a decrease in systolic blood pressure in presence of the fractionated peptides, being 100 mg/kg a dose comparable to Captopril (positive control). These results contribute to the current knowledge of bioactive peptides from quinoa by reporting the relevance of EDUF as tool to produce selected peptide fractions. Nevertheless, further characterization is needed towards peptide sequencing, their respective role in the metabolism and scaling-up production using EDUF.

Original language	English
Pages (from-to)	1650-1659
Number of pages	10
Journal	Food Science and Human Wellness
Volume	11
Issue number	6
DOIs	https://doi.org/10.1016/j.fshw.2022.06.024
State	Published - Nov 2022

Bibliographical note

Funding Information:
This project was financially supported by the Postdoctoral Fellowship Nº 3190683 of Dr. Adrián González-Muñoz from the Chilean Agencia Nacional de Investigación y Desarrollo (ANID) and the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant Program (Grant SD RGPIN-2018-04128 of Prof. Laurent Bazinet). The authors would like to specially thank Jacinthe Thibodeau from the Department of Food Sciences, Université Laval for her technical support.

Funding Information:
This project was financially supported by the Postdoctoral Fellowship Nº 3190683 of Dr. Adrián González-Muñoz from the Chilean Agencia Nacional de Investigación y Desarrollo (ANID) and the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant Program (Grant SD RGPIN-2018-04128 of Prof. Laurent Bazinet). The authors would like to specially thank Jacinthe Thibodeau from the Department of Food Sciences, Université Laval for her technical support.

Publisher Copyright:
© 2022

Keywords

Antidiabetic
Antihypertensive
Bioactive peptides
Electrodialysis with ultrafiltration membranes
Quinoa

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10.1016/j.fshw.2022.06.024

Cite this

@article{c21e3d0bb0b64a72a92be18a04cacfb5,

title = "Production of antihypertensive and antidiabetic peptide fractions from quinoa (Chenopodium quinoa Willd.) by electrodialysis with ultrafiltration membranes",

abstract = "Processing bioactive peptides from natural sources using electrodialysis with ultrafiltration membranes (EDUF) have gained attention since it can fractionate in terms of their charge and molecular weight. Quinoa is a pseudo-cereal highlighted by its high protein content, amino acid profile and adapting growing conditions. The present work aimed at the production of quinoa peptides through fractionation using EDUF and to test the fractions according to antihypertensive and antidiabetic activity. Experimental data showed the production of peptides ranging between 0.4 and 1.5 kDa. Cationic (CQPF) (3.01 %), anionic (AQPF) (1.18 %) and the electrically neutral fraction quinoa protein hydrolysate (QPH)-EDUF (∼95 %) were obtained. In-vitro studies showed the highest glucose uptake modulation in L6 cell skeletal myoblasts in presence of QPH-EDUF and AQPF (17 % and 11 %) indicating potential antidiabetic activity. The antihypertensive effect studied in-vivo in spontaneously hypertensive rats (SHR), showed a decrease in systolic blood pressure in presence of the fractionated peptides, being 100 mg/kg a dose comparable to Captopril (positive control). These results contribute to the current knowledge of bioactive peptides from quinoa by reporting the relevance of EDUF as tool to produce selected peptide fractions. Nevertheless, further characterization is needed towards peptide sequencing, their respective role in the metabolism and scaling-up production using EDUF.",

keywords = "Antidiabetic, Antihypertensive, Bioactive peptides, Electrodialysis with ultrafiltration membranes, Quinoa, Quinoa, Electrodialysis with ultrafiltration membranes, Bioactive peptides, Antihypertensive, Antidiabetic",

author = "Adri{\'a}n Gonz{\'a}lez-Mu{\~n}oz and Marion Valle and Aluko, {Rotimi E.} and Laurent Bazinet and Javier Enrione",

note = "Funding Information: This project was financially supported by the Postdoctoral Fellowship Nº 3190683 of Dr. Adri{\'a}n Gonz{\'a}lez-Mu{\~n}oz from the Chilean Agencia Nacional de Investigaci{\'o}n y Desarrollo (ANID) and the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant Program (Grant SD RGPIN-2018-04128 of Prof. Laurent Bazinet). The authors would like to specially thank Jacinthe Thibodeau from the Department of Food Sciences, Universit{\'e} Laval for her technical support. Funding Information: This project was financially supported by the Postdoctoral Fellowship Nº 3190683 of Dr. Adri{\'a}n Gonz{\'a}lez-Mu{\~n}oz from the Chilean Agencia Nacional de Investigaci{\'o}n y Desarrollo (ANID) and the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant Program (Grant SD RGPIN-2018-04128 of Prof. Laurent Bazinet). The authors would like to specially thank Jacinthe Thibodeau from the Department of Food Sciences, Universit{\'e} Laval for her technical support. Publisher Copyright: {\textcopyright} 2022",

year = "2022",

month = nov,

doi = "10.1016/j.fshw.2022.06.024",

language = "English",

volume = "11",

pages = "1650--1659",

journal = "Food Science and Human Wellness",

issn = "2213-4530",

publisher = "Elsevier BV",

number = "6",

}

TY - JOUR

T1 - Production of antihypertensive and antidiabetic peptide fractions from quinoa (Chenopodium quinoa Willd.) by electrodialysis with ultrafiltration membranes

AU - González-Muñoz, Adrián

AU - Valle, Marion

AU - Aluko, Rotimi E.

AU - Bazinet, Laurent

AU - Enrione, Javier

N1 - Funding Information: This project was financially supported by the Postdoctoral Fellowship Nº 3190683 of Dr. Adrián González-Muñoz from the Chilean Agencia Nacional de Investigación y Desarrollo (ANID) and the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant Program (Grant SD RGPIN-2018-04128 of Prof. Laurent Bazinet). The authors would like to specially thank Jacinthe Thibodeau from the Department of Food Sciences, Université Laval for her technical support. Funding Information: This project was financially supported by the Postdoctoral Fellowship Nº 3190683 of Dr. Adrián González-Muñoz from the Chilean Agencia Nacional de Investigación y Desarrollo (ANID) and the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant Program (Grant SD RGPIN-2018-04128 of Prof. Laurent Bazinet). The authors would like to specially thank Jacinthe Thibodeau from the Department of Food Sciences, Université Laval for her technical support. Publisher Copyright: © 2022

PY - 2022/11

Y1 - 2022/11

N2 - Processing bioactive peptides from natural sources using electrodialysis with ultrafiltration membranes (EDUF) have gained attention since it can fractionate in terms of their charge and molecular weight. Quinoa is a pseudo-cereal highlighted by its high protein content, amino acid profile and adapting growing conditions. The present work aimed at the production of quinoa peptides through fractionation using EDUF and to test the fractions according to antihypertensive and antidiabetic activity. Experimental data showed the production of peptides ranging between 0.4 and 1.5 kDa. Cationic (CQPF) (3.01 %), anionic (AQPF) (1.18 %) and the electrically neutral fraction quinoa protein hydrolysate (QPH)-EDUF (∼95 %) were obtained. In-vitro studies showed the highest glucose uptake modulation in L6 cell skeletal myoblasts in presence of QPH-EDUF and AQPF (17 % and 11 %) indicating potential antidiabetic activity. The antihypertensive effect studied in-vivo in spontaneously hypertensive rats (SHR), showed a decrease in systolic blood pressure in presence of the fractionated peptides, being 100 mg/kg a dose comparable to Captopril (positive control). These results contribute to the current knowledge of bioactive peptides from quinoa by reporting the relevance of EDUF as tool to produce selected peptide fractions. Nevertheless, further characterization is needed towards peptide sequencing, their respective role in the metabolism and scaling-up production using EDUF.

AB - Processing bioactive peptides from natural sources using electrodialysis with ultrafiltration membranes (EDUF) have gained attention since it can fractionate in terms of their charge and molecular weight. Quinoa is a pseudo-cereal highlighted by its high protein content, amino acid profile and adapting growing conditions. The present work aimed at the production of quinoa peptides through fractionation using EDUF and to test the fractions according to antihypertensive and antidiabetic activity. Experimental data showed the production of peptides ranging between 0.4 and 1.5 kDa. Cationic (CQPF) (3.01 %), anionic (AQPF) (1.18 %) and the electrically neutral fraction quinoa protein hydrolysate (QPH)-EDUF (∼95 %) were obtained. In-vitro studies showed the highest glucose uptake modulation in L6 cell skeletal myoblasts in presence of QPH-EDUF and AQPF (17 % and 11 %) indicating potential antidiabetic activity. The antihypertensive effect studied in-vivo in spontaneously hypertensive rats (SHR), showed a decrease in systolic blood pressure in presence of the fractionated peptides, being 100 mg/kg a dose comparable to Captopril (positive control). These results contribute to the current knowledge of bioactive peptides from quinoa by reporting the relevance of EDUF as tool to produce selected peptide fractions. Nevertheless, further characterization is needed towards peptide sequencing, their respective role in the metabolism and scaling-up production using EDUF.

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KW - Antihypertensive

KW - Bioactive peptides

KW - Electrodialysis with ultrafiltration membranes

KW - Quinoa

KW - Electrodialysis with ultrafiltration membranes

KW - Bioactive peptides

KW - Antihypertensive

KW - Antidiabetic

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U2 - 10.1016/j.fshw.2022.06.024

DO - 10.1016/j.fshw.2022.06.024

M3 - Article

AN - SCOPUS:85134571046

SN - 2213-4530

VL - 11

SP - 1650

EP - 1659

JO - Food Science and Human Wellness

JF - Food Science and Human Wellness

IS - 6

ER -

Production of antihypertensive and antidiabetic peptide fractions from quinoa (Chenopodium quinoa Willd.) by electrodialysis with ultrafiltration membranes

Abstract

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Keywords

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