TY - JOUR
T1 - Probiotic screening and safety evaluation of lactobacillus strains from plants, artisanal goat cheese, human stools, and breast milk
AU - Gotteland, Martin
AU - Cires, Maria Jose
AU - Carvallo, Claudia
AU - Vega, Natalia
AU - Ramirez, Maria Antonieta
AU - Morales, Pamela
AU - Rivas, Patricia
AU - Astudillo, Fernanda
AU - Navarrete, Paola
AU - Dubos, Céline
AU - Figueroa, Alvaro
AU - Troncoso, Miriam
AU - Ulloa, Carolina
AU - Mizgier, Maria Luisa
AU - Carrasco-Pozo, Catalina
AU - Speisky, Hernan
AU - Brunser, Oscar
AU - Figueroa, Guillermo
PY - 2014/4/1
Y1 - 2014/4/1
N2 - The aim of this study was to select autochthonous strains of Lactobacillus from stools of healthy infants and adults, human milk, artisanal goat cheese, and fruits and vegetables according to their probiotic properties and safety. From 421 strains of Lactobacillus isolated, 102 (24.2%) were shown to be tolerant to gastric pH and bile salts; they were used to determine their anti-Helicobacter pylori (agar diffusion assay), antioxidant (oxygen radical absorption capacity), and anti-inflammatory (inhibition of interleukin-8 release by tumor necrosis factor-α-stimulated HT-29 cells) activities as well as their ability to adhere to intestinal (Caco-2) and gastric (AGS) epithelial cells. Results obtained were compared with three commercial probiotic Lactobacillus rhamnosus GG, L. plantarum 299v, and L. johnsonii NCC533. The five strains most efficient according to these activities were subsequently identified by sequencing their 16S rRNA gene, their susceptibility to antibiotics was determined, and their safety evaluated in mice. One strain of L. plantarum was discarded due to the higher prevalence of liver bacterial translocation observed in the animals fed this strain. In conclusion, four autochthonous strains of L. rhamnosus were finally selected with probiotic properties and safety allowing their eventual use in human studies. These results contribute to increase the diversity of probiotic strains available for the development of nutraceuticals and functional foods.
AB - The aim of this study was to select autochthonous strains of Lactobacillus from stools of healthy infants and adults, human milk, artisanal goat cheese, and fruits and vegetables according to their probiotic properties and safety. From 421 strains of Lactobacillus isolated, 102 (24.2%) were shown to be tolerant to gastric pH and bile salts; they were used to determine their anti-Helicobacter pylori (agar diffusion assay), antioxidant (oxygen radical absorption capacity), and anti-inflammatory (inhibition of interleukin-8 release by tumor necrosis factor-α-stimulated HT-29 cells) activities as well as their ability to adhere to intestinal (Caco-2) and gastric (AGS) epithelial cells. Results obtained were compared with three commercial probiotic Lactobacillus rhamnosus GG, L. plantarum 299v, and L. johnsonii NCC533. The five strains most efficient according to these activities were subsequently identified by sequencing their 16S rRNA gene, their susceptibility to antibiotics was determined, and their safety evaluated in mice. One strain of L. plantarum was discarded due to the higher prevalence of liver bacterial translocation observed in the animals fed this strain. In conclusion, four autochthonous strains of L. rhamnosus were finally selected with probiotic properties and safety allowing their eventual use in human studies. These results contribute to increase the diversity of probiotic strains available for the development of nutraceuticals and functional foods.
KW - anti-inflammatory activity
KW - antimicrobial activity
KW - antioxidant capacity
KW - Lactobacillus
KW - probiotics
UR - http://www.scopus.com/inward/record.url?scp=84922554366&partnerID=8YFLogxK
U2 - 10.1089/jmf.2013.0030
DO - 10.1089/jmf.2013.0030
M3 - Article
C2 - 24433075
AN - SCOPUS:84922554366
SN - 1096-620X
VL - 17
SP - 487
EP - 495
JO - Journal of Medicinal Food
JF - Journal of Medicinal Food
IS - 4
ER -