Participation of S. Typhimurium cysJIH operon in the H2S-mediated ciprofloxacin resistance in presence of sulfate as sulfur source

Ricardo Álvarez; Jorge Frávega; Paula I. Rodas; Juan A. Fuentes; Daniel Paredes-Sabja; Iván L. Calderón; Fernando Gil

doi:10.3390/antibiotics4030321

Participation of S. Typhimurium _cysJIH operon in the H₂S-mediated ciprofloxacin resistance in presence of sulfate as sulfur source

Ricardo Álvarez
, Jorge Frávega
, Paula I. Rodas
, Juan A. Fuentes
, Daniel Paredes-Sabja
, Iván L. Calderón
, Fernando Gil^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

H₂S production has been proposed as a mechanism to explain bacterial resistance to antibiotics. In this work, we present evidence for the role of the cysJIH operon in resistance to ciprofloxacin mediated by H₂S production with different sulfate as the only sulfur source. We found that the products of the cysJIH operon are involved in ciprofloxacin resistance by increasing both, the levels of H₂S and reduced thiols apparently counteracting antimicrobial-induced reactive oxygen species (ROS). This protective effect was observed only when bacteria were cultured in the presence of sulfate, but not with cysteine, as the sole sulfur source.

Original language	English
Pages (from-to)	321-328
Number of pages	8
Journal	Antibiotics
Volume	4
Issue number	3
DOIs	https://doi.org/10.3390/antibiotics4030321
State	Published - 31 Jul 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015, by the authors.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

JIH
Ciprofloxacin resistance
HS production
Sulfate
Sulfur source

Access to Document

10.3390/antibiotics4030321

Cite this

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title = "Participation of S. Typhimurium cysJIH operon in the H2S-mediated ciprofloxacin resistance in presence of sulfate as sulfur source",

abstract = "H2S production has been proposed as a mechanism to explain bacterial resistance to antibiotics. In this work, we present evidence for the role of the cysJIH operon in resistance to ciprofloxacin mediated by H2S production with different sulfate as the only sulfur source. We found that the products of the cysJIH operon are involved in ciprofloxacin resistance by increasing both, the levels of H2S and reduced thiols apparently counteracting antimicrobial-induced reactive oxygen species (ROS). This protective effect was observed only when bacteria were cultured in the presence of sulfate, but not with cysteine, as the sole sulfur source.",

keywords = "JIH, Ciprofloxacin resistance, HS production, Sulfate, Sulfur source",

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note = "Publisher Copyright: {\textcopyright} 2015, by the authors.",

year = "2015",

month = jul,

day = "31",

doi = "10.3390/antibiotics4030321",

language = "English",

volume = "4",

pages = "321--328",

journal = "Antibiotics",

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}

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T1 - Participation of S. Typhimurium cysJIH operon in the H2S-mediated ciprofloxacin resistance in presence of sulfate as sulfur source

AU - Álvarez, Ricardo

AU - Frávega, Jorge

AU - Rodas, Paula I.

AU - Fuentes, Juan A.

AU - Paredes-Sabja, Daniel

AU - Calderón, Iván L.

AU - Gil, Fernando

PY - 2015/7/31

Y1 - 2015/7/31

N2 - H2S production has been proposed as a mechanism to explain bacterial resistance to antibiotics. In this work, we present evidence for the role of the cysJIH operon in resistance to ciprofloxacin mediated by H2S production with different sulfate as the only sulfur source. We found that the products of the cysJIH operon are involved in ciprofloxacin resistance by increasing both, the levels of H2S and reduced thiols apparently counteracting antimicrobial-induced reactive oxygen species (ROS). This protective effect was observed only when bacteria were cultured in the presence of sulfate, but not with cysteine, as the sole sulfur source.

AB - H2S production has been proposed as a mechanism to explain bacterial resistance to antibiotics. In this work, we present evidence for the role of the cysJIH operon in resistance to ciprofloxacin mediated by H2S production with different sulfate as the only sulfur source. We found that the products of the cysJIH operon are involved in ciprofloxacin resistance by increasing both, the levels of H2S and reduced thiols apparently counteracting antimicrobial-induced reactive oxygen species (ROS). This protective effect was observed only when bacteria were cultured in the presence of sulfate, but not with cysteine, as the sole sulfur source.

KW - JIH

KW - Ciprofloxacin resistance

KW - HS production

KW - Sulfate

KW - Sulfur source

UR - https://www.scopus.com/pages/publications/85019815925

U2 - 10.3390/antibiotics4030321

DO - 10.3390/antibiotics4030321

M3 - Article

AN - SCOPUS:85019815925

SN - 2079-6382

VL - 4

SP - 321

EP - 328

JO - Antibiotics

JF - Antibiotics

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