Overexpression of heat-shock protein 47 impacts survival of patients with oral squamous cell carcinoma

Bruno Cesar da Costa; Mauricio Rocha Dourado; Everton Freitas de Moraes; Luana Marí Panini; Amr Elseragy; Fábio Haach Téo; Gustavo Narvaes Guimarães; Renato Assis Machado; Maija Risteli; Clarissa Araujo Gurgel Rocha; Lívia Máris Ribeiro Paranaíba; Wilfredo Alejandro González-Arriagada; Sabrina Daniela da Silva; Ana Lucia Carrinho Ayroza Rangel; Marcelo Rocha Marques; Tuula Salo; Ricardo D. Coletta

doi:10.1111/jop.13455

Overexpression of heat-shock protein 47 impacts survival of patients with oral squamous cell carcinoma

Bruno Cesar da Costa, Mauricio Rocha Dourado, Everton Freitas de Moraes, Luana Marí Panini, Amr Elseragy, Fábio Haach Téo, Gustavo Narvaes Guimarães, Renato Assis Machado, Maija Risteli, Clarissa Araujo Gurgel Rocha, Lívia Máris Ribeiro Paranaíba, Wilfredo Alejandro González-Arriagada, Sabrina Daniela da Silva, Ana Lucia Carrinho Ayroza Rangel, Marcelo Rocha Marques, Tuula Salo, Ricardo D. Coletta^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

BACKGROUND: The expression of heat-shock protein 47 (HSP47) has been linked to collagen synthesis control and implicated in fibrotic disorders, but more recent studies have demonstrated its role in solid tumors. In this study, we explored the prognostic impact of HSP47 in oral squamous cell carcinomas (OSCC) and determined the in vitro effects of its loss-of-function on viability, proliferation, migration, invasion, and resistance to cisplatin of OSCC cells.

METHODS: The HSP47 expression in tumor samples was assessed by immunohistochemistry in two independent cohorts totaling 339 patients with OSCC, and protein levels were associated with clinicopathological features and survival outcomes. The OSCC cell lines HSC3 and SCC9 were transduced with lentivirus expressing short hairpin RNA to stably silence HSP47 and used in assays to measure cellular viability, proliferation, migration, and invasion.

RESULTS: HSP47 was overexpressed in OSCC samples, and its overexpression was significantly and independently associated with poor disease-specific survival and shortened disease-free survival in both OSCC cohorts. The knockdown of HSP47 showed no effects on cell viability or cisplatin sensitivity, but impaired significantly proliferation, migration, and invasion of OSCC cells, with stronger effects on SCC9 cells.

CONCLUSION: Our results show a significant prognostic impact of HSP47 overexpression in OSCC and reveal that HSP47 inhibition impairs the proliferation, migration, and invasion of OSCC cells. HSP47 may represent a potential therapeutic target for OSCC.

Original language	English
Pages (from-to)	601-609
Number of pages	9
Journal	Journal of Oral Pathology and Medicine
Volume	52
Issue number	7
Early online date	29 May 2023
DOIs	https://doi.org/10.1111/jop.13455
State	Published - Aug 2023

Bibliographical note

Publisher Copyright:
© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Keywords

SERPINH1
invasion
oral cancer
prognosis
proliferation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/jop.13455

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da Costa, B. C., Dourado, M. R., de Moraes, E. F., Panini, L. M., Elseragy, A., Téo, F. H., Guimarães, G. N., Machado, R. A., Risteli, M., Gurgel Rocha, C. A., Paranaíba, L. M. R., González-Arriagada, W. A., da Silva, S. D., Rangel, A. L. C. A., Marques, M. R., Salo, T., & Coletta, R. D. (2023). Overexpression of heat-shock protein 47 impacts survival of patients with oral squamous cell carcinoma. Journal of Oral Pathology and Medicine, 52(7), 601-609. https://doi.org/10.1111/jop.13455

@article{efaf956a557444fc96747089ca99361f,

title = "Overexpression of heat-shock protein 47 impacts survival of patients with oral squamous cell carcinoma",

abstract = "BACKGROUND: The expression of heat-shock protein 47 (HSP47) has been linked to collagen synthesis control and implicated in fibrotic disorders, but more recent studies have demonstrated its role in solid tumors. In this study, we explored the prognostic impact of HSP47 in oral squamous cell carcinomas (OSCC) and determined the in vitro effects of its loss-of-function on viability, proliferation, migration, invasion, and resistance to cisplatin of OSCC cells.METHODS: The HSP47 expression in tumor samples was assessed by immunohistochemistry in two independent cohorts totaling 339 patients with OSCC, and protein levels were associated with clinicopathological features and survival outcomes. The OSCC cell lines HSC3 and SCC9 were transduced with lentivirus expressing short hairpin RNA to stably silence HSP47 and used in assays to measure cellular viability, proliferation, migration, and invasion.RESULTS: HSP47 was overexpressed in OSCC samples, and its overexpression was significantly and independently associated with poor disease-specific survival and shortened disease-free survival in both OSCC cohorts. The knockdown of HSP47 showed no effects on cell viability or cisplatin sensitivity, but impaired significantly proliferation, migration, and invasion of OSCC cells, with stronger effects on SCC9 cells.CONCLUSION: Our results show a significant prognostic impact of HSP47 overexpression in OSCC and reveal that HSP47 inhibition impairs the proliferation, migration, and invasion of OSCC cells. HSP47 may represent a potential therapeutic target for OSCC.",

keywords = "SERPINH1, invasion, oral cancer, prognosis, proliferation",

author = "{da Costa}, {Bruno Cesar} and Dourado, {Mauricio Rocha} and {de Moraes}, {Everton Freitas} and Panini, {Luana Mar{\'i}} and Amr Elseragy and T{\'e}o, {F{\'a}bio Haach} and Guimar{\~a}es, {Gustavo Narvaes} and Machado, {Renato Assis} and Maija Risteli and {Gurgel Rocha}, {Clarissa Araujo} and Parana{\'i}ba, {L{\'i}via M{\'a}ris Ribeiro} and Gonz{\'a}lez-Arriagada, {Wilfredo Alejandro} and {da Silva}, {Sabrina Daniela} and Rangel, {Ana Lucia Carrinho Ayroza} and Marques, {Marcelo Rocha} and Tuula Salo and Coletta, {Ricardo D.}",

note = "Publisher Copyright: {\textcopyright} 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.",

year = "2023",

month = aug,

doi = "10.1111/jop.13455",

language = "English",

volume = "52",

pages = "601--609",

journal = "Journal of Oral Pathology and Medicine",

issn = "0904-2512",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "7",

}

da Costa, BC, Dourado, MR, de Moraes, EF, Panini, LM, Elseragy, A, Téo, FH, Guimarães, GN, Machado, RA, Risteli, M, Gurgel Rocha, CA, Paranaíba, LMR, González-Arriagada, WA, da Silva, SD, Rangel, ALCA, Marques, MR, Salo, T & Coletta, RD 2023, 'Overexpression of heat-shock protein 47 impacts survival of patients with oral squamous cell carcinoma', Journal of Oral Pathology and Medicine, vol. 52, no. 7, pp. 601-609. https://doi.org/10.1111/jop.13455

TY - JOUR

T1 - Overexpression of heat-shock protein 47 impacts survival of patients with oral squamous cell carcinoma

AU - da Costa, Bruno Cesar

AU - Dourado, Mauricio Rocha

AU - de Moraes, Everton Freitas

AU - Panini, Luana Marí

AU - Elseragy, Amr

AU - Téo, Fábio Haach

AU - Guimarães, Gustavo Narvaes

AU - Machado, Renato Assis

AU - Risteli, Maija

AU - Gurgel Rocha, Clarissa Araujo

AU - Paranaíba, Lívia Máris Ribeiro

AU - González-Arriagada, Wilfredo Alejandro

AU - da Silva, Sabrina Daniela

AU - Rangel, Ana Lucia Carrinho Ayroza

AU - Marques, Marcelo Rocha

AU - Salo, Tuula

AU - Coletta, Ricardo D.

PY - 2023/8

Y1 - 2023/8

N2 - BACKGROUND: The expression of heat-shock protein 47 (HSP47) has been linked to collagen synthesis control and implicated in fibrotic disorders, but more recent studies have demonstrated its role in solid tumors. In this study, we explored the prognostic impact of HSP47 in oral squamous cell carcinomas (OSCC) and determined the in vitro effects of its loss-of-function on viability, proliferation, migration, invasion, and resistance to cisplatin of OSCC cells.METHODS: The HSP47 expression in tumor samples was assessed by immunohistochemistry in two independent cohorts totaling 339 patients with OSCC, and protein levels were associated with clinicopathological features and survival outcomes. The OSCC cell lines HSC3 and SCC9 were transduced with lentivirus expressing short hairpin RNA to stably silence HSP47 and used in assays to measure cellular viability, proliferation, migration, and invasion.RESULTS: HSP47 was overexpressed in OSCC samples, and its overexpression was significantly and independently associated with poor disease-specific survival and shortened disease-free survival in both OSCC cohorts. The knockdown of HSP47 showed no effects on cell viability or cisplatin sensitivity, but impaired significantly proliferation, migration, and invasion of OSCC cells, with stronger effects on SCC9 cells.CONCLUSION: Our results show a significant prognostic impact of HSP47 overexpression in OSCC and reveal that HSP47 inhibition impairs the proliferation, migration, and invasion of OSCC cells. HSP47 may represent a potential therapeutic target for OSCC.

AB - BACKGROUND: The expression of heat-shock protein 47 (HSP47) has been linked to collagen synthesis control and implicated in fibrotic disorders, but more recent studies have demonstrated its role in solid tumors. In this study, we explored the prognostic impact of HSP47 in oral squamous cell carcinomas (OSCC) and determined the in vitro effects of its loss-of-function on viability, proliferation, migration, invasion, and resistance to cisplatin of OSCC cells.METHODS: The HSP47 expression in tumor samples was assessed by immunohistochemistry in two independent cohorts totaling 339 patients with OSCC, and protein levels were associated with clinicopathological features and survival outcomes. The OSCC cell lines HSC3 and SCC9 were transduced with lentivirus expressing short hairpin RNA to stably silence HSP47 and used in assays to measure cellular viability, proliferation, migration, and invasion.RESULTS: HSP47 was overexpressed in OSCC samples, and its overexpression was significantly and independently associated with poor disease-specific survival and shortened disease-free survival in both OSCC cohorts. The knockdown of HSP47 showed no effects on cell viability or cisplatin sensitivity, but impaired significantly proliferation, migration, and invasion of OSCC cells, with stronger effects on SCC9 cells.CONCLUSION: Our results show a significant prognostic impact of HSP47 overexpression in OSCC and reveal that HSP47 inhibition impairs the proliferation, migration, and invasion of OSCC cells. HSP47 may represent a potential therapeutic target for OSCC.

KW - SERPINH1

KW - invasion

KW - oral cancer

KW - prognosis

KW - proliferation

UR - http://www.scopus.com/inward/record.url?scp=85161033862&partnerID=8YFLogxK

U2 - 10.1111/jop.13455

DO - 10.1111/jop.13455

M3 - Article

C2 - 37247331

SN - 0904-2512

VL - 52

SP - 601

EP - 609

JO - Journal of Oral Pathology and Medicine

JF - Journal of Oral Pathology and Medicine

IS - 7

ER -

Overexpression of heat-shock protein 47 impacts survival of patients with oral squamous cell carcinoma

Abstract

Bibliographical note

Keywords

UN SDGs

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