Ovarian cholesterol efflux: ATP-binding cassette transporters and follicular fluid HDL regulate cholesterol content in mouse oocytes

Alonso Quiroz, Paz Molina, Nicolás Santander, Daniel Gallardo, Attilio Rigotti, Dolores Busso*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

High density lipoproteins (HDL) take up cholesterol from peripheral tissues via ABC transporters and deliver it to the liver via scavenger receptor class B type I (SR-B1). HDL are the main lipoproteins present in follicular fluid (FF). They are thought to derive from plasma, but their origin is still controversial. SR-B1 knock-out (KO) mice have provided important evidence linking HDL metabolism and female fertility. These mice have cholesterol-rich circulating HDL and female infertility that can be restored by treating mice with the cholesterol-lowering drug probucol. Ovulated oocytes from SR-B1 KO females are dysfunctional and show excess cholesterol. The mechanisms explaining the contribution of FF HDL to oocyte cholesterol homeostasis are unknown. Here, using quantitation of filipin fluorescence we show that in SR-B1 KO ovaries, cholesterol excess is first observed in immature oocytes in antral follicles. By performing cross-transplant experiments between WT and apolipoprotein A-I deficient (ApoA-I KO) mice, which lack the main protein component of HDL, we provide evidence supporting the plasmatic origin of FF HDL. Also, we demonstrate that probucol treatment in SR-B1 KO females results in lowering of cholesterol content in their oocytes. Incubation of oocytes from SR-B1 KO mice with purified WT HDL reduces their cholesterol content, suggesting that HDL promote efflux of excess cholesterol from oocytes. In agreement with this hypothesis, we identified ABC transporters in oocytes and observed that ABCA1 KO oocytes have excess cholesterol and lower viability than WT oocytes.

Original languageEnglish
Pages (from-to)348-361
Number of pages14
JournalBiology of Reproduction
Volume102
Issue number2
DOIs
StatePublished - 14 Feb 2020
Externally publishedYes

Bibliographical note

Funding Information:
†Grant Support: Chilean National Council for Scientific and Technological Research (CONICYT) program Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT) through grants #1141236 and #1180347 (to DB) and #1150399 (to AR). CONICYT PhD Fellowship #21170306 (to AQ) and #21130444 (to NS). Undergraduate Student Fellowship from the Research Direction, School of Medicine, Pontificia Universidad Católica de Chile (to DG).

Publisher Copyright:
© 2019 The Author(s). Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved.

Keywords

  • fertility
  • follicular maturation
  • oocyte

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