Oral polio vaccine in infants does not interfere in detection of enterovirus in blood

Marcela González, Carmen Sandoval, Patricia Valenzuela, Luisa Montecinos, Constanza Martínez, Paula Godoy, Katia Abarca*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction: There is not known if a viraemia post-oral polio vaccine (OPV) is detectable by modern molecular techniques. Such viraemia could affect the performance of the real time-polymerase chain reaction (PCR) for non polio enterovirus (EV) detection, technique of growing clinical use for the study of febrile infants. Objective: To determine viraemia post-first dose of OPV in healthy infants, by molecular techniques. Patients and Methods: 50 infants less than three months without previous VPO were randomized in 5 groups: a control group with prevaccination blood sample (BS), group 1 BS at day 2, group 2 BS at day 4, group 3, BS at day 6 and group 4, BS at day 8 post-vaccination. Conventional and specific PCR for poliovirus and real time PCR for non polio EV were performed in BS and in OPV samples. Results: No genetic material of poliovirus was detected in any infant, while in 9 of them (18%) non polio EV was identified. Real time PCR for EV did not amplify poliovirus from OPV samples. Discussion: Results suggest that no post VPO viraemia detectable by molecular methods exists. Considering that real time PCR for EV does not allow to identify polio virus, no false positives of the test are expected as a result of a recent VPO vaccination. We documented presence of non polio EV in blood of healthy asymptomatic infants.

Translated title of the contributionLa vacuna polio oral en lactantes no interfiere con la detección de enterovirus en sangre
Original languageEnglish
Pages (from-to)626-629
Number of pages4
JournalRevista Chilena de Infectologia
Volume30
Issue number6
DOIs
StatePublished - Dec 2013
Externally publishedYes

Keywords

  • Enterovirus
  • PCR
  • Poliovirus vaccine viraemia
  • Real time PCR

Fingerprint

Dive into the research topics of 'Oral polio vaccine in infants does not interfere in detection of enterovirus in blood'. Together they form a unique fingerprint.

Cite this