New ependymal cells are born postnatally in two discrete regions of the mouse brain and support ventricular enlargement in hydrocephalus

Luis Federico Batiz, Antonio J. Jiménez, Montserrat Guerra, Luis Manuel Rodríguez-Pérez, César D. Toledo, Karin Vio, Patricia Páez, José Manuel Pérez-Fígares, Esteban M. Rodríguez

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

A heterogeneous population of ependymal cells lines the brain ventricles. The evidence about the origin and birth dates of these cell populations is scarce. Furthermore, the possibility that mature ependymal cells are born (ependymogenesis) or self-renewed (ependymal proliferation) postnatally is controversial. The present study was designed to investigate both phenomena in wild-type (wt) and hydrocephalic α-SNAP mutant (hyh) mice at different postnatal stages. In wt mice, proliferating cells in the ventricular zone (VZ) were only found in two distinct regions: the dorsal walls of the third ventricle and Sylvian aqueduct (SA). Most proliferating cells were monociliated and nestin+, likely corresponding to radial glial cells. Postnatal cumulative BrdU-labeling showed that most daughter cells remained in the VZ of both regions and they lost nestin-immunoreactivity. Furthermore, some labeled cells became multiciliated and GLUT-1+, indicating they were ependymal cells born postnatally. Postnatal pulse BrdU-labeling and Ki-67 immunostaining further demonstrated the presence of cycling multiciliated ependymal cells. In hydrocephalic mutants, the dorsal walls of the third ventricle and SA expanded enormously and showed neither ependymal disruption nor ventriculostomies. This phenomenon was sustained by an increased ependymogenesis. Consequently, in addition to the physical and geometrical mechanisms traditionally explaining ventricular enlargement in fetal-onset hydrocephalus, we propose that postnatal ependymogenesis could also play a role. Furthermore, as generation of new ependymal cells during postnatal stages was observed in distinct regions of the ventricular walls, such as the roof of the third ventricle, it may be a key mechanism involved in the development of human type 1 interhemispheric cysts.

Original languageEnglish
Pages (from-to)721-735
Number of pages15
JournalActa Neuropathologica
Volume121
Issue number6
DOIs
StatePublished - Jun 2011
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgments We thank Mr. Genaro Alvial for his valuable technical support. This work was supported by Grants from Fondecyt-Chile 1070241 to EMR and 11090373 to LFB; PCI2006-A/-0669 and PS09/00376, Instituto de Salud Carlos III and Servicio Andaluz de Salud, Spain to AJJ.

Keywords

  • Ependyma
  • Hydrocephalus
  • Postnatal ependymogenesis
  • Proliferation
  • Radial glia
  • Type 1 interhemispheric cyst

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