Molecular signatures of extracellular vesicles in oral fluids of periodontitis patients

Alejandra Chaparro Padilla*, Laura Weber Aracena, Ornella Realini Fuentes, Daniela Albers Busquetts, Marcela Hernández Ríos, Valeria Ramírez Lobos, Andrés Pascual La Rocca, José Nart Molina, Victor Beltrán Varas, Stephanie Acuña-Gallardo, Antonio Sanz Ruiz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Objective: To characterize extracellular vesicles (EVs) in gingival crevicular fluid (GCF) and saliva samples from healthy/gingivitis and periodontitis patients and correlate them with clinical inflammatory periodontal parameters. Material and Method: An exploratory study, including 86 subjects, was conducted. Clinical and periodontal data were recorded, and oral fluid samples were obtained. EVs were precipitated by ExoQuick-TC™ and characterized by nanoparticle tracking (NanoSight™), Western blot (WB), transmission electron microscopy (TEM), and ELISA analysis. Results: TEM showed nanoparticles morphologically compatible with EVs, and WB analysis revealed bands of specific EV markers (CD9, TSG101, and Alix) in both oral fluids of periodontitis and healthy/gingivitis subjects. The total concentration of EVs in GCF was increased in periodontitis patients compared to healthy/gingivitis subjects (p =.017). However, we did not observe differences in the EV concentration of saliva samples (p =.190). The size of GCF-EVs was 144.2 nm in periodontitis and 160.35 nm in healthy/gingivitis patients (p =.038). The CD63 exosome marker was increased in GCF of periodontitis patients (p =.00001). The total concentration of EVs in GCF was correlated with bleeding on probing (rho = 0.63, p =.002), periodontal probing depth (rho = 0.56, p =.009), and clinical attachment level (rho = 0.48, p =.030). Conclusion: Periodontitis patients have an increased concentration of EVs in GCF, and their role in periodontitis should be clarified.

Original languageEnglish
Pages (from-to)1318-1325
Number of pages8
JournalOral Diseases
Volume26
Issue number6
DOIs
StatePublished - 1 Sep 2020

Bibliographical note

Publisher Copyright:
© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved

Keywords

  • exosomes
  • extracellular vesicles
  • micro-vesicles
  • periodontitis

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