Molecular Biomarkers in Peri-Implant Health and Disease: A Cross-Sectional Pilot Study

Alejandra Chaparro, Víctor Beltrán*, Daniel Betancur, Ye Han Sam, Haniyeh Moaven, Ali Tarjomani, Nikolaos Donos, Vanessa Sousa

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Background: The aim of this feasibility study was to investigate the concentration level of CCL-20/MIP-3α, BAFF/BLyS, IL-23, RANKL, and Osteoprotegerin in the Peri-Implant Crevicular Fluid (PICF), from patients diagnosed with peri-implant mucositis and peri-implantitis, and to compare them with PICF from patients with healthy implants. Methods: Participants with at least one dental implant with healthy peri-implant tissues, peri-implant mucositis, or peri-implantitis were included. PICF was collected using paper strips from healthy and diseased peri-implant sites (n = 19). Biomarker levels were analyzed using a custom Multiplex ELISA Assay Kit. Results: In comparison to peri-implant health, the peri-implant mucositis group showed an increased concentration of CCL-20 MIP-3α, BAFF/BLyS, IL-23, RANKL, and Osteoprotegerin. The peri-implantitis group had the lowest median concentration of Osteoprotegerin (1963 ng/mL); this group had a similar concentration of RANKL (640.84 ng/mL) when compared to the peri-implant health group. BAFF/BLyS (17.06 ng/mL) showed the highest concentration in the peri-implantitis group. Conclusions: This feasibility study suggests that IL-23 and RANKL may help to elucidate the pathogenesis during the conversion from peri-implant health to peri-implantitis. Further research is required in BAFF/BLyS for the early diagnosis of peri-implantitis.

Original languageEnglish
Article number9802
JournalInternational Journal of Molecular Sciences
Issue number17
StatePublished - 29 Aug 2022

Bibliographical note

Publisher Copyright:
© 2022 by the authors.


  • Biomarkers/analysis
  • Cross-Sectional Studies
  • Dental Implants/adverse effects
  • Gingival Crevicular Fluid
  • Humans
  • Interleukin-23
  • Mucositis
  • Osteoprotegerin/analysis
  • Peri-Implantitis/diagnosis
  • Pilot Projects


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