Molecular Biomarkers in Peri-Implant Health and Disease: A Cross-Sectional Pilot Study

Alejandra Chaparro; Víctor Beltrán; Daniel Betancur; Ye Han Sam; Haniyeh Moaven; Ali Tarjomani; Nikolaos Donos; Vanessa Sousa

doi:10.3390/ijms23179802

Molecular Biomarkers in Peri-Implant Health and Disease: A Cross-Sectional Pilot Study

Alejandra Chaparro, Víctor Beltrán^*, Daniel Betancur, Ye Han Sam, Haniyeh Moaven, Ali Tarjomani, Nikolaos Donos, Vanessa Sousa

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Background: The aim of this feasibility study was to investigate the concentration level of CCL-20/MIP-3α, BAFF/BLyS, IL-23, RANKL, and Osteoprotegerin in the Peri-Implant Crevicular Fluid (PICF), from patients diagnosed with peri-implant mucositis and peri-implantitis, and to compare them with PICF from patients with healthy implants. Methods: Participants with at least one dental implant with healthy peri-implant tissues, peri-implant mucositis, or peri-implantitis were included. PICF was collected using paper strips from healthy and diseased peri-implant sites (n = 19). Biomarker levels were analyzed using a custom Multiplex ELISA Assay Kit. Results: In comparison to peri-implant health, the peri-implant mucositis group showed an increased concentration of CCL-20 MIP-3α, BAFF/BLyS, IL-23, RANKL, and Osteoprotegerin. The peri-implantitis group had the lowest median concentration of Osteoprotegerin (1963 ng/mL); this group had a similar concentration of RANKL (640.84 ng/mL) when compared to the peri-implant health group. BAFF/BLyS (17.06 ng/mL) showed the highest concentration in the peri-implantitis group. Conclusions: This feasibility study suggests that IL-23 and RANKL may help to elucidate the pathogenesis during the conversion from peri-implant health to peri-implantitis. Further research is required in BAFF/BLyS for the early diagnosis of peri-implantitis.

Original language	English
Article number	9802
Journal	International Journal of Molecular Sciences
Volume	23
Issue number	17
DOIs	https://doi.org/10.3390/ijms23179802
State	Published - 29 Aug 2022

Bibliographical note

Funding Information:
This research was funded by National Agency for Research and Development of Chile (ANID), Project No. REDI170658. D.B. was supported by the School of Dentistry, Universidad de Concepción, Concepción, Chile. V.S. was supported by the National Institute for Health Research of the United Kingdom.

Publisher Copyright:
© 2022 by the authors.

Keywords

Biomarkers/analysis
Cross-Sectional Studies
Dental Implants/adverse effects
Gingival Crevicular Fluid
Humans
Interleukin-23
Mucositis
Osteoprotegerin/analysis
Peri-Implantitis/diagnosis
Pilot Projects

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10.3390/ijms23179802

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@article{17f794b1954d4384b799b4e5c00b5bef,

title = "Molecular Biomarkers in Peri-Implant Health and Disease: A Cross-Sectional Pilot Study",

abstract = "Background: The aim of this feasibility study was to investigate the concentration level of CCL-20/MIP-3α, BAFF/BLyS, IL-23, RANKL, and Osteoprotegerin in the Peri-Implant Crevicular Fluid (PICF), from patients diagnosed with peri-implant mucositis and peri-implantitis, and to compare them with PICF from patients with healthy implants. Methods: Participants with at least one dental implant with healthy peri-implant tissues, peri-implant mucositis, or peri-implantitis were included. PICF was collected using paper strips from healthy and diseased peri-implant sites (n = 19). Biomarker levels were analyzed using a custom Multiplex ELISA Assay Kit. Results: In comparison to peri-implant health, the peri-implant mucositis group showed an increased concentration of CCL-20 MIP-3α, BAFF/BLyS, IL-23, RANKL, and Osteoprotegerin. The peri-implantitis group had the lowest median concentration of Osteoprotegerin (1963 ng/mL); this group had a similar concentration of RANKL (640.84 ng/mL) when compared to the peri-implant health group. BAFF/BLyS (17.06 ng/mL) showed the highest concentration in the peri-implantitis group. Conclusions: This feasibility study suggests that IL-23 and RANKL may help to elucidate the pathogenesis during the conversion from peri-implant health to peri-implantitis. Further research is required in BAFF/BLyS for the early diagnosis of peri-implantitis.",

keywords = "Biomarkers/analysis, Cross-Sectional Studies, Dental Implants/adverse effects, Gingival Crevicular Fluid, Humans, Interleukin-23, Mucositis, Osteoprotegerin/analysis, Peri-Implantitis/diagnosis, Pilot Projects",

author = "Alejandra Chaparro and V{\'i}ctor Beltr{\'a}n and Daniel Betancur and Sam, {Ye Han} and Haniyeh Moaven and Ali Tarjomani and Nikolaos Donos and Vanessa Sousa",

note = "Funding Information: This research was funded by National Agency for Research and Development of Chile (ANID), Project No. REDI170658. D.B. was supported by the School of Dentistry, Universidad de Concepci{\'o}n, Concepci{\'o}n, Chile. V.S. was supported by the National Institute for Health Research of the United Kingdom. Publisher Copyright: {\textcopyright} 2022 by the authors.",

year = "2022",

month = aug,

day = "29",

doi = "10.3390/ijms23179802",

language = "English",

volume = "23",

journal = "International Journal of Molecular Sciences",

issn = "1661-6596",

publisher = "MDPI Multidisciplinary Digital Publishing Institute",

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TY - JOUR

T1 - Molecular Biomarkers in Peri-Implant Health and Disease

T2 - A Cross-Sectional Pilot Study

AU - Chaparro, Alejandra

AU - Beltrán, Víctor

AU - Betancur, Daniel

AU - Sam, Ye Han

AU - Moaven, Haniyeh

AU - Tarjomani, Ali

AU - Donos, Nikolaos

AU - Sousa, Vanessa

N1 - Funding Information: This research was funded by National Agency for Research and Development of Chile (ANID), Project No. REDI170658. D.B. was supported by the School of Dentistry, Universidad de Concepción, Concepción, Chile. V.S. was supported by the National Institute for Health Research of the United Kingdom. Publisher Copyright: © 2022 by the authors.

PY - 2022/8/29

Y1 - 2022/8/29

N2 - Background: The aim of this feasibility study was to investigate the concentration level of CCL-20/MIP-3α, BAFF/BLyS, IL-23, RANKL, and Osteoprotegerin in the Peri-Implant Crevicular Fluid (PICF), from patients diagnosed with peri-implant mucositis and peri-implantitis, and to compare them with PICF from patients with healthy implants. Methods: Participants with at least one dental implant with healthy peri-implant tissues, peri-implant mucositis, or peri-implantitis were included. PICF was collected using paper strips from healthy and diseased peri-implant sites (n = 19). Biomarker levels were analyzed using a custom Multiplex ELISA Assay Kit. Results: In comparison to peri-implant health, the peri-implant mucositis group showed an increased concentration of CCL-20 MIP-3α, BAFF/BLyS, IL-23, RANKL, and Osteoprotegerin. The peri-implantitis group had the lowest median concentration of Osteoprotegerin (1963 ng/mL); this group had a similar concentration of RANKL (640.84 ng/mL) when compared to the peri-implant health group. BAFF/BLyS (17.06 ng/mL) showed the highest concentration in the peri-implantitis group. Conclusions: This feasibility study suggests that IL-23 and RANKL may help to elucidate the pathogenesis during the conversion from peri-implant health to peri-implantitis. Further research is required in BAFF/BLyS for the early diagnosis of peri-implantitis.

AB - Background: The aim of this feasibility study was to investigate the concentration level of CCL-20/MIP-3α, BAFF/BLyS, IL-23, RANKL, and Osteoprotegerin in the Peri-Implant Crevicular Fluid (PICF), from patients diagnosed with peri-implant mucositis and peri-implantitis, and to compare them with PICF from patients with healthy implants. Methods: Participants with at least one dental implant with healthy peri-implant tissues, peri-implant mucositis, or peri-implantitis were included. PICF was collected using paper strips from healthy and diseased peri-implant sites (n = 19). Biomarker levels were analyzed using a custom Multiplex ELISA Assay Kit. Results: In comparison to peri-implant health, the peri-implant mucositis group showed an increased concentration of CCL-20 MIP-3α, BAFF/BLyS, IL-23, RANKL, and Osteoprotegerin. The peri-implantitis group had the lowest median concentration of Osteoprotegerin (1963 ng/mL); this group had a similar concentration of RANKL (640.84 ng/mL) when compared to the peri-implant health group. BAFF/BLyS (17.06 ng/mL) showed the highest concentration in the peri-implantitis group. Conclusions: This feasibility study suggests that IL-23 and RANKL may help to elucidate the pathogenesis during the conversion from peri-implant health to peri-implantitis. Further research is required in BAFF/BLyS for the early diagnosis of peri-implantitis.

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KW - Gingival Crevicular Fluid

KW - Humans

KW - Interleukin-23

KW - Mucositis

KW - Osteoprotegerin/analysis

KW - Peri-Implantitis/diagnosis

KW - Pilot Projects

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U2 - 10.3390/ijms23179802

DO - 10.3390/ijms23179802

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SN - 1661-6596

VL - 23

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

IS - 17

M1 - 9802

ER -

Molecular Biomarkers in Peri-Implant Health and Disease: A Cross-Sectional Pilot Study

Abstract

Bibliographical note

Keywords

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