Molecular Biomarkers in Peri-Implant Health and Disease: A Cross-Sectional Pilot Study

Alejandra Chaparro, Víctor Beltrán*, Daniel Betancur, Ye Han Sam, Haniyeh Moaven, Ali Tarjomani, Nikolaos Donos, Vanessa Sousa

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Background: The aim of this feasibility study was to investigate the concentration level of CCL-20/MIP-3α, BAFF/BLyS, IL-23, RANKL, and Osteoprotegerin in the Peri-Implant Crevicular Fluid (PICF), from patients diagnosed with peri-implant mucositis and peri-implantitis, and to compare them with PICF from patients with healthy implants. Methods: Participants with at least one dental implant with healthy peri-implant tissues, peri-implant mucositis, or peri-implantitis were included. PICF was collected using paper strips from healthy and diseased peri-implant sites (n = 19). Biomarker levels were analyzed using a custom Multiplex ELISA Assay Kit. Results: In comparison to peri-implant health, the peri-implant mucositis group showed an increased concentration of CCL-20 MIP-3α, BAFF/BLyS, IL-23, RANKL, and Osteoprotegerin. The peri-implantitis group had the lowest median concentration of Osteoprotegerin (1963 ng/mL); this group had a similar concentration of RANKL (640.84 ng/mL) when compared to the peri-implant health group. BAFF/BLyS (17.06 ng/mL) showed the highest concentration in the peri-implantitis group. Conclusions: This feasibility study suggests that IL-23 and RANKL may help to elucidate the pathogenesis during the conversion from peri-implant health to peri-implantitis. Further research is required in BAFF/BLyS for the early diagnosis of peri-implantitis.

Original languageEnglish
Article number9802
JournalInternational Journal of Molecular Sciences
Issue number17
StatePublished - 29 Aug 2022

Bibliographical note

Funding Information:
This research was funded by National Agency for Research and Development of Chile (ANID), Project No. REDI170658. D.B. was supported by the School of Dentistry, Universidad de Concepción, Concepción, Chile. V.S. was supported by the National Institute for Health Research of the United Kingdom.

Publisher Copyright:
© 2022 by the authors.


  • Biomarkers/analysis
  • Cross-Sectional Studies
  • Dental Implants/adverse effects
  • Gingival Crevicular Fluid
  • Humans
  • Interleukin-23
  • Mucositis
  • Osteoprotegerin/analysis
  • Peri-Implantitis/diagnosis
  • Pilot Projects


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