Mmp-8, trap-5, and opg levels in gcf diagnostic potential to discriminate between healthy patients’, mild and severe periodontitis sites

Marcela Hernández, Mauricio Baeza, Johanna Contreras, Timo Sorsa, Taina Tervahartiala, Macarena Valdés, Alejandra Chaparro, Patricia Hernández-Ríos*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Biomarkers represent promising aids in periodontitis, host-mediate diseases of the tooth-supporting tissues. We assessed the diagnostic potential of matrix metalloproteinase-8 (MMP-8), tartrate-resistant acid phosphatase-5 (TRAP-5), and osteoprotegerin (OPG) to discriminate between healthy patients’, mild and severe periodontitis sites. Thirty-one otherwise healthy volunteers with and without periodontal disease were enrolled at the Faculty of Dentistry, University of Chile. Periodontal parameters were examined and gingival crevicular fluid was sampled from mild periodontitis sites (M; n = 42), severe periodontitis sites (S; n = 59), and healthy volunteer sites (H; n = 30). TRAP-5 and OPG were determined by commercial multiplex assay and MMP-8 by the immunofluorometric (IFMA) method. STATA software was used. All biomarkers showed a good discrimination performance. MMP-8 had the overall best performance in regression models and Receiver Operating Characteristic (ROC) curves, with high discrimination of healthy from periodontitis sites (area under the curve (AUC) = 0.901). OPG showed a very high diagnostic precision (AUC ≥ 0.95) to identify severe periodontitis sites (S versus H + M), while TRAP-5 identified both healthy and severe sites. As conclusions, MMP-8, TRAP-5, and OPG present a high precision potential in the identification of periodontal disease destruction, with MMP-8 as the most accurate diagnostic biomarker.

Original languageEnglish
Article number1500
Pages (from-to)1-14
Number of pages14
Issue number11
StatePublished - Nov 2020

Bibliographical note

Funding Information:
Funding: This research was funded by FONDECYT 1090741, 1090046 and 1200098, Chilean government; the University of Helsinki Research Foundation, Helsinki, Finland (grant numbers TYH2016251, TYH2017251, TYH2018229, TYH2019319, Y1014SL017, Y1014SL018, and Y1014SULE1), the Finnish Dental Society Apollonia and the Karolinska Institutet, Stockholm, Sweden. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.


  • Biomarkers
  • Gingival crevicular fluid
  • Matrix metalloproteinase-8
  • Osteoprotegerin
  • Periodontitis
  • Tartrate-resistant acid phosphatase


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