Low-Level Laser Irradiation Promotes Proliferation and Differentiation on Apical Papilla Stem Cells

David Gutiérrez, Mahmoud Rouabhia, Javiera Ortiz, Diego Gaviria, Camilo Alfonso, Ana Muñoz, Carolina Inostroza*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction: Low-level laser therapy (LLLT) has been reported to improve cell proliferation and differentiation. The stem cells derived from dental apical papilla (SCAPs) are a promising therapy because they are easily obtained from immature human teeth. The effect of LLLT over SCAPs is still unknown. This study aimed to evaluate the proliferation and osteogenic potential of the SCAPs stimulated with LLLT. Methods: SCAPs were isolated from the third molars of a healthy donor and characterized according to the minimum established criteria. SCAPs were cultured for 24 hours before being exposed to LLLT. Cells were exposed to different doses, energy, and wavelengths for selecting the irradiation parameters. SCAPs proliferation was evaluated with the MTT assay at 24 hours and 7-day post-laser exposure. VEGF and TGFβ2 expression were assessed with a specific enzyme-linked immunosorbent assay (ELISA). The osteogenic differentiation potential was analyzed with alizarin red staining, and the nodule quantification was performed by the relative optical density (ROD) analysis using ImageJ software. Results: The cells isolated from the apical papilla showed phenotype and stem cell properties. SCAPs irradiated with one dose at 6 J/m2 and 650 nm exhibited significantly higher proliferation (P > 0.05) than the controls nonirradiated. LLLT stimulated SCAPs’ expression of factors VEGF and TGFβ2. Also, SCAPs irradiated showed higher osteogenic activity (P < 0.05). Conclusion: LLLT promotes proliferation, osteogenic differentiation, and VEGF and TGFβ2 expression on SCAPs. LLLT is a practical approach for the preconditioning of SCAPs in vitro for future regenerative therapies. More studies are needed to determine the underlying molecular processes that determine the mechanism of the LLLT.

Original languageEnglish
Article numbere75
JournalJournal of Lasers in Medical Sciences
Volume12
Issue number1
DOIs
StatePublished - 2021

Bibliographical note

Funding Information:
This work was supported by funds from the Vice-rectory of Science Technology and Research (VCTI) of the Universidad Antonio Nari?o (Grants 2018209). David Gutierrez was supported by the Program of Formation of High Level (PFAN) of the Universidad Antonio Nari?o, and the Sistema Nacional de Regal?as (SGR) de Colombia and by the project INNOVACCION Cauca.

Funding Information:
This work was supported by funds from the Vice-rectory of Science Technology and Research (VCTI) of the Universidad Antonio Nariño (Grants 2018209). David Gutierrez was supported by the Program of Formation of High Level (PFAN) of the Universidad Antonio Nariño, and the Sistema Nacional de Regalías (SGR) de Colombia and by the project INNOVACCION Cauca.

Publisher Copyright:
© 2021. Journal of Lasers in Medical Sciences. All Rights Reserved.

Keywords

  • Apical papilla stem cells
  • Cell differentiation
  • Cell proliferation
  • Low-level laser therapy

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