We have previously reported the detection of endotoxin in the amniotic fluid of patients with gram-negative intraamniotic infection. Endotoxin or lipopolysaccharide is a potent biologic product capable of inducing prostaglandin release from several cell types and, therefore, may be involved in the onset of human parturition in the presence of intraamniotic infection. This article describes a technique for the quantification of endotoxin in amniotic fluid. The method uses a computer-assisted quantification of the turbidimetric reaction between the Limulus amebocyte lysate and endotoxin. Serial dilutions of Escherichia coli endotoxin in culture-negative amniotic fluid were prepared, and the samples were run in the assay. Amniotic fluid was found to enhance the reaction, and a dilution of 1:20 was required for this biologic fluid to behave similarly to pyrogen-free water. The sensitivity of this kinetic turbidimetric technique in the detection of endotoxin in amniotic fluid was 40 pg/ml. This method was applied to the quantification of endotoxin concentration in amniotic fluid in 26 patients with intraamniotic infection and premature rupture of membranes. Patients in active labor had higher concentrations of endotoxin (median = 47,514 pg/ml) than nonlaboring patients (median = 635 pg/ml) (p < 0.025). Therefore, women with preterm labor had a higher median concentration of endotoxin in amniotic fluid than patients who were not in labor
Bibliographical noteFunding Information:
From the Section of Maternal-Fetal Medicine, the Department of Obstetrics and Gynecology, Yale University School of Medicine, and Associates of Cape Cod, Inc. This work was supported by a grant from the Walter Scott Foundation for Medical Research. Received for publication july 20, 1987; accepted November 18, 1987. Reprint requests: Roberto Romero, MD, Yale University School of Medicine, Department of Obstetrics and Gynecology, 333 Cedar St., P. 0. Box 3333, New Haven, CT 06510-8063. *Recipient of a Physician Scientist Award from theN ational Institutes of Health.
- Intraamniotic infection
- Limulus amebocyte lysate
- turbidimetric technique