Intrauterine growth restriction modifies the normal gene expression in kidney from rabbit fetuses

Horacio Figueroa; Mauricio Lozano; Cristian Suazo; Elisenda Eixarch; Sebastian E. Illanes; Juan Eduardo Carreño; Sandra Villanueva; Edgar Hernández-Andrade; Eduard Gratacós; Carlos E. Irarrazabal

doi:10.1016/j.earlhumdev.2012.07.010

Intrauterine growth restriction modifies the normal gene expression in kidney from rabbit fetuses

Horacio Figueroa, Mauricio Lozano, Cristian Suazo, Elisenda Eixarch, Sebastian E. Illanes, Juan Eduardo Carreño, Sandra Villanueva, Edgar Hernández-Andrade, Eduard Gratacós, Carlos E. Irarrazabal

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The aim of this work was to study the effect of intrauterine growth restriction (IUGR) on fetal kidneys. The IUGR was induced by uteroplacental vessels ligature in a model of pregnant rabbit. We centralized the study in the gene expression of essential proteins for fetal kidney development and kidney protection against hypoxia, osmotic stress, and kidney injury. The gene expression of HIF-1α, NFAT5, IL-1β, NGAL, and ATM were studied by qRT-PCR and Western blot in kidneys from control and IUGR fetuses. Experimental IUGR fetuses were significantly smaller than the control animals (39 vs. 48. g, p < 0.05). The number of glomeruli was decreased in IUGR kidneys, without morphological alterations. IUGR increased the gene expression of HIF-1α, NFAT5, IL-1β, NGAL, and ATM (p < 0.05) in kidneys of fetuses undergoing IUGR, suggesting that fetal blood flow restriction produce alterations in gene expression in fetal kidneys.

Original language	American English
Pages (from-to)	899-904
Number of pages	6
Journal	Early Human Development
Volume	88
Issue number	11
DOIs	https://doi.org/10.1016/j.earlhumdev.2012.07.010
State	Published - 1 Nov 2012

Keywords

Hypoxia
Inflammation
IUGR
Osmotic stress
Rabbit

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Figueroa, Horacio ; Lozano, Mauricio ; Suazo, Cristian ; Eixarch, Elisenda ; Illanes, Sebastian E. ; Carreño, Juan Eduardo ; Villanueva, Sandra ; Hernández-Andrade, Edgar ; Gratacós, Eduard ; Irarrazabal, Carlos E. / Intrauterine growth restriction modifies the normal gene expression in kidney from rabbit fetuses. In: Early Human Development. 2012 ; Vol. 88, No. 11. pp. 899-904.

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title = "Intrauterine growth restriction modifies the normal gene expression in kidney from rabbit fetuses",

abstract = "The aim of this work was to study the effect of intrauterine growth restriction (IUGR) on fetal kidneys. The IUGR was induced by uteroplacental vessels ligature in a model of pregnant rabbit. We centralized the study in the gene expression of essential proteins for fetal kidney development and kidney protection against hypoxia, osmotic stress, and kidney injury. The gene expression of HIF-1α, NFAT5, IL-1β, NGAL, and ATM were studied by qRT-PCR and Western blot in kidneys from control and IUGR fetuses. Experimental IUGR fetuses were significantly smaller than the control animals (39 vs. 48. g, p < 0.05). The number of glomeruli was decreased in IUGR kidneys, without morphological alterations. IUGR increased the gene expression of HIF-1α, NFAT5, IL-1β, NGAL, and ATM (p < 0.05) in kidneys of fetuses undergoing IUGR, suggesting that fetal blood flow restriction produce alterations in gene expression in fetal kidneys.",

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author = "Horacio Figueroa and Mauricio Lozano and Cristian Suazo and Elisenda Eixarch and Illanes, {Sebastian E.} and Carre{\~n}o, {Juan Eduardo} and Sandra Villanueva and Edgar Hern{\'a}ndez-Andrade and Eduard Gratac{\'o}s and Irarrazabal, {Carlos E.}",

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Intrauterine growth restriction modifies the normal gene expression in kidney from rabbit fetuses. / Figueroa, Horacio; Lozano, Mauricio; Suazo, Cristian; Eixarch, Elisenda; Illanes, Sebastian E.; Carreño, Juan Eduardo; Villanueva, Sandra; Hernández-Andrade, Edgar; Gratacós, Eduard; Irarrazabal, Carlos E.

In: Early Human Development, Vol. 88, No. 11, 01.11.2012, p. 899-904.

Research output: Contribution to journal › Article › peer-review

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