Interlukin-1 stimulates prostaglandin biosynthesis by human amnion

R. Romero*, S. Durum, C. A. Dinarello, E. Oyarzun, J. C. Hobbins, M. D. Mitchell

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

279 Scopus citations

Abstract

The purpose of these studies was to determine if Interleukin-1 (IL-1) alters the rate of prostaglandin biosynthesis by human amnion. Primary monolayer cultures of amnion cells were established from women undergoing elective cesarean section before the onset of labor. Natural purified and recombinant human IL-1α and IL-1α were incubated with amnion cells in culture, and prostaglandin E2 (PGE2) biosynthesis was measured by radioimmunoassay in cell-free media. A concentration-dependent increase in PGE2 production by amnion cells occurred in response to natural purified and recombinant IL-1 preparations. No differences in the parameters of the dose-response curves between the two IL-1 gene products could be determined (p > 0.05). Indomethacin blocked the effectof IL-1 in prostaglandin biosynthesis by human amnion. Interleukin-1, a fever mediator, could serve as a signal for the initiation of labor in cases of intrauterine or systemic infection.

Original languageEnglish
Pages (from-to)13-22
Number of pages10
JournalProstaglandins
Volume37
Issue number1
DOIs
StatePublished - Jan 1989
Externally publishedYes

Bibliographical note

Funding Information:
Supported by grants from the Walter Scott Foundation for Medical Research and NIH Grants HD 20779 and Al 15614. Dr. Romero is the recipient of a Physician Scientist Award grant from the National Institutes of Child Health and Human Development.

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