Injectable and release-controlled OP-1 hybrid core-shell nanocapsules enhance distraction osteogenesis in rabbits

Ziyad Haidar, R. C. Hamdy, M. Tabrizian

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

A bone morphogenetic protein-7 (BMP-7, also known as osteogenic protein 1 or OP-1) delivery system for potential de novo bone tissue regeneration and acceleration in distraction osteogenesis (DO) is presented. A liposomal core was incorporated in a shell of alternating layer-by-layer (L-b-L) self-assembled natural polyelectrolytes; alginate and chitosan. Hydrophilic, non-toxic, monodisperse, spherical and stable cationic capsules with an extended shelf-life allowing immediate protein loading prior to clinical administration were formulated. Protein encapsulation and release from the nanocapsules can be modulated by modifying the number of polymer layers assembled on the liposomal core. Controlled, triphasic, linear and sustained release of bioactive OP-1 was observed, demonstrating the core-shell effect, in vitro. In vivo, un-loaded and OP-1 loaded nanocapsules were evaluated in a rabbit model of tibial DO. Regenerate structure analysis followed. Osteogenesis and consolidation were accelerated via a single injection of the core-shell nanocapsules loaded with a dose of no more than 1.0 μg OP-1 accentuating further the role of the hybrid nanocapsules. Hence, a promising and cost-effective carrier for the localized and release-controlled administration of therapeutic growth factors has been formulated.

Original languageEnglish
Title of host publication25th Southern Biomedical Engineering Conference 2009
Pages355-358
Number of pages4
DOIs
StatePublished - 2009
Externally publishedYes
Event25th Southern Biomedical Engineering Conference 2009 - Miami, FL, United States
Duration: 15 May 200917 May 2009

Publication series

NameIFMBE Proceedings
Volume24
ISSN (Print)1680-0737

Conference

Conference25th Southern Biomedical Engineering Conference 2009
CountryUnited States
CityMiami, FL
Period15/05/0917/05/09

Keywords

  • BMP-7
  • Core-shell delivery system
  • Distraction osteogenesis
  • In vitro and in vivo assessments

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