Increased free fetal DNA levels in early pregnancy plasma of women who subsequently develop preeclampsia and intrauterine growth restriction

S. Illanes, M. Parra, R. Serra, K. Pino, H. Figueroa-Diesel, C. Romero, J. A. Arraztoa, L. Michea, P. W. Soothill

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48 Scopus citations

Abstract

Objective: To determine if maternal plasma ffDNA is increased early in pregnancies which subsequently develop preeclampsia (PE) and intrauterine growth restriction (IUGR). Methods: Blood was obtained at 11-14 weeks and plasma stored. Among those who delivered a male infant and had a birth weight under the tenth centile and/or PE, we divided them into those who delivered before 35 weeks (9) and those who delivered after this gestation (15). A third group with uncomplicated pregnancies was used as controls (24). Real time-polymerase chain reaction (RT-PCR) was carried out to detect the multi-copy Y chromosome associated DSY14 gene. Results: There were no differences between the ffDNA levels in the group delivered after 35 weeks and the control group (2.23ge/mL-1.61ge/mL p = 0.39). However, the levels of ffDNA at 11-14 weeks were statistically, significantly higher in patients that delivered before 35 weeks (4.34ge/mL-1.61ge/mL p = 0.0018). A logistic regression analysis shows that for every unit (1ge/mL) in which ffDNA increases, the likelihood of having PE or a fetus growing under the tenth centile delivered before 35 weeks increases by 1.67 times (CI 1.13-2.47). Conclusion: The concentration of ffDNA is significantly higher even during early pregnancy, in patients who subsequently develop PE and/or IUGR and are delivered before 35 weeks. Copyright
Original languageAmerican English
Pages (from-to)1118-1122
Number of pages5
JournalPrenatal Diagnosis
Volume29
Issue number12
DOIs
StatePublished - 1 Dec 2009

Keywords

  • DNA
  • Fetal and placental pathology
  • Fetal cells
  • Maternal disease
  • Maternal serum screening
  • Nucleic acids and proteins
  • Placental disease
  • Preeclampsia

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