The present study aims to evaluate antimicrobial activity, cytotoxicity, as well as resin-dentine microtensile bond strength, nanoleakage and degree of conversion of six universal adhesives, on sound and caries-affected dentine after 18 months. The adhesives Prime&Bond Active (PBA), Scotchbond Universal (SBU), Tetric N-Bond Universal (TNU), Ambar Universal (AMU), Clearfil Universal Bond Quick (CUQ) and One Coat 7 Universal (OCU) were used. Antimicrobial activity was evaluated against Streptococcus mutans. For cytotoxicity, methyltetrazolium assay was used, after 24 h exposure of osteoblast-like cells line to the adhesive's dilution of 1, 0.1, and 0.01 v/v%. After, the adhesives were applied in etch-and-rinse or self-etch strategies on sound or caries-affected dentine surfaces, resin composites restorations were constructed. Then, the specimens were sectioned to obtain sticks to be evaluated in microtensile bond strength and nanoleakage after 24 h and 18 months, and degree of conversion after 24 h. ANOVA and Tukey's test were applied (α = 0.05). For antimicrobial activity, CUQ showed higher values than all adhesives. For cytotoxicity, the PBA, AMU, CUQ and OCU adhesives presented cytotoxicity in different dilutions. For microtensile bond strength, OCU presented the lowest values, regardless of time, dentine or strategy. For nanoleakage, differences were observed among adhesives depending on time, dentine or strategy. For degree of conversion, TNU presented the highest values, while PBA and OCU presented the lowest values. Worst values of microtensile bond strength and nanoleakage were always obtained in caries-affected dentine after 18 months. Thus, not all universal adhesives behave the same in terms of antimicrobial activity and cytotoxicity. However, the majority showed worst results when applied in caries-affected dentine, mainly after 18 months. Clinical relevance. Universal adhesive systems may have differences in their biological and adhesive properties, both on sound, but mainly in caries-affected dentine after 18 months.
|Journal||International Journal of Adhesion and Adhesives|
|State||Published - Apr 2022|
Bibliographical noteFunding Information:
This study was performed by Mario Felipe Guti?rrez Reyes as partial fulfillment of his PhD degree at the State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil. This project was supported by Fondecyt (Fondo Nacional de Desarrollo Cient?fico y Tecnol?gico - Chile) [project 1,170,575 (Chile; EF)]; partially supported by the National Council for Scientific and Technological Development (CNPq) [305,588/2014-1] (Brazil; ADL); and in part by the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - Brasil (CAPES) - Finance Code 001.
This study was performed by Mario Felipe Gutiérrez Reyes as partial fulfillment of his PhD degree at the State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil. This project was supported by Fondecyt ( Fondo Nacional de Desarrollo Científico y Tecnológico - Chile ) [project 1,170,575 (Chile; EF)]; partially supported by the National Council for Scientific and Technological Development (CNPq) [305,588/2014-1] (Brazil; ADL); and in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.
- Hybrid layer
- Mechanical properties of adhesives
- Universal adhesive system