IL-17A levels increase in the infarcted region of the left ventricle in a rat model of myocardial infarction

Ana María Ávalos, Felipe A. Apablaza, Mariana Quiroz, Viviana Toledo, Juan Pedro Peña, Luis Michea, Carlos Ernesto Irarrazabal Muñoz, Flavio A. Carrión, Fernando Figueroa

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Th17 cells, a recently described subtype of CD4+ effector lymphocytes, have been linked to cell-mediated autoimmune and inflammatory diseases as well as to cardiovascular diseases. However, the participation of IL-17A in myocardial ischemic injury has not been clearly defined. We therefore conducted the present study to evaluate IL-17A and Th17-related cytokine levels in a rat model of myocardial infarction (MI). MI was induced in male Sprague Dawley rats by coronary artery ligation. Controls were sham-operated (Sh) or non-operated (C). Blood and samples from the left ventricle (LV) were collected at weeks 1 and 4 post-MI. At week 1, MI animals exhibited increased IL-6, IL-23 and TGF-β mRNA levels with no apparent change in IL-17 mRNA or protein levels in whole LV. Only TGF-β mRNA remained elevated at week 4 post-MI. However, further analysis revealed that IL-17A mRNA and protein levels as well as IL-6 and IL-23 mRNA were indeed increased in the infarcted region, though not in the remote non infarcted region of the LV, except for IL-23 mRNA. The increased expression of IL-17A and Th17-related cytokines in the infarcted region of LV, suggests that this proinflammatory pathway might play a role in early stages of post MI cardiac remodelling.

Original languageEnglish
Pages (from-to)193-200
Number of pages8
JournalBiological Research
Volume45
Issue number2
DOIs
StatePublished - 2012

Keywords

  • Cardiovascular disease
  • Interleukin-17
  • Myocardial infarction
  • Th17

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