Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein

Daniela Vittori; Graciela Garbossa; Carlos Lafourcade; Gladys Pérez; Alcira Nesse

doi:10.1016/S0005-2736(01)00427-8

Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein

Daniela Vittori^*, Graciela Garbossa, Carlos Lafourcade, Gladys Pérez, Alcira Nesse

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

There is evidence that anaemia is associated with aluminium (Al). We have already reported on the sensitivity to Al, showed by erythroid cell populations of animals chronically exposed to the metal. In order to investigate whether Al could also affect human cells, experiments were carried out both on immature and mature human erythroid cells. Erythroid progenitors (CFU-E, colony-forming units-erythroid) concentrated from human peripheral blood were cultured in an Al-rich medium under erythropoietin stimulation and their development analysed. Human peripheral erythrocytes were aged in the presence of Al. Cells were examined using scanning electron microscopy, and membrane proteins analysed by polyacrylamide gel electrophoresis with sodium dodecyl sulphate and immunoblotting. The development of the Al-treated progenitors was 8750/6600-9200 CFU-E/10⁶ cells, a significantly lower median value (P<0.05) than that showed by non-treated cells (12 300/11 200-20 700 CFU-E/10⁶ cells). Erythrocyte morphological changes were induced by Al during the in vitro ageing. The cells lost their typical biconcave shape, turning into acanthocytes and stomatocytes. Simultaneously, an increased membrane protein breakdown compatible with band 3 degradation was detected. Besides, Al was found within the cells and attached to the membrane. The present in vitro results suggest that Al may disturb human erythropoiesis through combined effects on mature erythrocytes and cellular metabolism in late erythroid progenitors.

Original language	English
Pages (from-to)	142-150
Number of pages	9
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	1558
Issue number	2
DOIs	https://doi.org/10.1016/S0005-2736(01)00427-8
State	Published - 1 Feb 2002
Externally published	Yes

Bibliographical note

Funding Information:
We are grateful to Dr. Jorge Stripeikis for measuring Al levels, Mr. Juan Pablo Vittori for his helpful assistance on photography, and Dr. Héctor Ostrowski for kindly supplying the Epo Pronivel (Elea S.A.C.I.F. y A., Argentina). This research was supported by grants from the Universidad de Buenos Aires, Consejo Nacional de Investigaciones Cientı́ficas y Técnicas (CONICET, Argentina) and Agencia Nacional de Promoción Cientı́fica y Tecnológica (ANPCYT, Argentina). Lic. D.Vittori has a fellowship from CONICET to fulfil her Ph.D. degree. Results of this work were partially presented at the XLV Annual Scientific Meeting, Sociedad Argentina de Investigación Clı́nica, Mar del Plata, Argentina (November 2000) and at the Fourth Keele Meeting on Aluminium, Stoke-on-Trent, United Kingdom (February 2001).

Keywords

Aluminium toxicity
Anaemia
Band 3
Erythrocyte morphology
Membrane proteins

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10.1016/S0005-2736(01)00427-8

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title = "Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein",

abstract = "There is evidence that anaemia is associated with aluminium (Al). We have already reported on the sensitivity to Al, showed by erythroid cell populations of animals chronically exposed to the metal. In order to investigate whether Al could also affect human cells, experiments were carried out both on immature and mature human erythroid cells. Erythroid progenitors (CFU-E, colony-forming units-erythroid) concentrated from human peripheral blood were cultured in an Al-rich medium under erythropoietin stimulation and their development analysed. Human peripheral erythrocytes were aged in the presence of Al. Cells were examined using scanning electron microscopy, and membrane proteins analysed by polyacrylamide gel electrophoresis with sodium dodecyl sulphate and immunoblotting. The development of the Al-treated progenitors was 8750/6600-9200 CFU-E/106 cells, a significantly lower median value (P<0.05) than that showed by non-treated cells (12 300/11 200-20 700 CFU-E/106 cells). Erythrocyte morphological changes were induced by Al during the in vitro ageing. The cells lost their typical biconcave shape, turning into acanthocytes and stomatocytes. Simultaneously, an increased membrane protein breakdown compatible with band 3 degradation was detected. Besides, Al was found within the cells and attached to the membrane. The present in vitro results suggest that Al may disturb human erythropoiesis through combined effects on mature erythrocytes and cellular metabolism in late erythroid progenitors.",

keywords = "Aluminium toxicity, Anaemia, Band 3, Erythrocyte morphology, Membrane proteins",

author = "Daniela Vittori and Graciela Garbossa and Carlos Lafourcade and Gladys P{\'e}rez and Alcira Nesse",

note = "Funding Information: We are grateful to Dr. Jorge Stripeikis for measuring Al levels, Mr. Juan Pablo Vittori for his helpful assistance on photography, and Dr. H{\'e}ctor Ostrowski for kindly supplying the Epo Pronivel (Elea S.A.C.I.F. y A., Argentina). This research was supported by grants from the Universidad de Buenos Aires, Consejo Nacional de Investigaciones Cientı́ficas y T{\'e}cnicas (CONICET, Argentina) and Agencia Nacional de Promoci{\'o}n Cientı́fica y Tecnol{\'o}gica (ANPCYT, Argentina). Lic. D.Vittori has a fellowship from CONICET to fulfil her Ph.D. degree. Results of this work were partially presented at the XLV Annual Scientific Meeting, Sociedad Argentina de Investigaci{\'o}n Clı́nica, Mar del Plata, Argentina (November 2000) and at the Fourth Keele Meeting on Aluminium, Stoke-on-Trent, United Kingdom (February 2001).",

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AU - Vittori, Daniela

AU - Garbossa, Graciela

AU - Lafourcade, Carlos

AU - Pérez, Gladys

AU - Nesse, Alcira

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N2 - There is evidence that anaemia is associated with aluminium (Al). We have already reported on the sensitivity to Al, showed by erythroid cell populations of animals chronically exposed to the metal. In order to investigate whether Al could also affect human cells, experiments were carried out both on immature and mature human erythroid cells. Erythroid progenitors (CFU-E, colony-forming units-erythroid) concentrated from human peripheral blood were cultured in an Al-rich medium under erythropoietin stimulation and their development analysed. Human peripheral erythrocytes were aged in the presence of Al. Cells were examined using scanning electron microscopy, and membrane proteins analysed by polyacrylamide gel electrophoresis with sodium dodecyl sulphate and immunoblotting. The development of the Al-treated progenitors was 8750/6600-9200 CFU-E/106 cells, a significantly lower median value (P<0.05) than that showed by non-treated cells (12 300/11 200-20 700 CFU-E/106 cells). Erythrocyte morphological changes were induced by Al during the in vitro ageing. The cells lost their typical biconcave shape, turning into acanthocytes and stomatocytes. Simultaneously, an increased membrane protein breakdown compatible with band 3 degradation was detected. Besides, Al was found within the cells and attached to the membrane. The present in vitro results suggest that Al may disturb human erythropoiesis through combined effects on mature erythrocytes and cellular metabolism in late erythroid progenitors.

AB - There is evidence that anaemia is associated with aluminium (Al). We have already reported on the sensitivity to Al, showed by erythroid cell populations of animals chronically exposed to the metal. In order to investigate whether Al could also affect human cells, experiments were carried out both on immature and mature human erythroid cells. Erythroid progenitors (CFU-E, colony-forming units-erythroid) concentrated from human peripheral blood were cultured in an Al-rich medium under erythropoietin stimulation and their development analysed. Human peripheral erythrocytes were aged in the presence of Al. Cells were examined using scanning electron microscopy, and membrane proteins analysed by polyacrylamide gel electrophoresis with sodium dodecyl sulphate and immunoblotting. The development of the Al-treated progenitors was 8750/6600-9200 CFU-E/106 cells, a significantly lower median value (P<0.05) than that showed by non-treated cells (12 300/11 200-20 700 CFU-E/106 cells). Erythrocyte morphological changes were induced by Al during the in vitro ageing. The cells lost their typical biconcave shape, turning into acanthocytes and stomatocytes. Simultaneously, an increased membrane protein breakdown compatible with band 3 degradation was detected. Besides, Al was found within the cells and attached to the membrane. The present in vitro results suggest that Al may disturb human erythropoiesis through combined effects on mature erythrocytes and cellular metabolism in late erythroid progenitors.

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KW - Band 3

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Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein

Abstract

Bibliographical note

Keywords

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