Human decidua: A source of interleukin-1

Roberto Romero*, Ying King Wu, Dan T. Brody, Enrique Oyarzun, Gordon W. Duff, Scott K. Durum

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

201 Scopus citations

Abstract

These studies were conducted to determine whether human decidua produces interleukin-1 in response to bacterial endotoxin. Explants of human decidua were incubated with and without Escherichia coli endotoxin for 20 hours. When tested for interleukin-1-Iike activity with the D10.G4.1 Tcell bioassay, supernatants from endotoxin-stimulated decidua contained significantly more interleukin-1 activity than did supernatants from unstimulated decidua. This activity could not be attributed to interleukin-2, as determined in the CTLL/2 assay for interleukin-2. Interleukin-1-like activity was due to interleukin-1, as demonstrated by the blockade of this bioactivity with antibodies against interleukin-1: interleukin-i alpha and interleukin-1 beta. Antibodies against interleukin-1 alpha blocked the activity in five of six cases. In one instance, the bioactivity could be attributed to a mixture of interleukin-1 alpha and interleukin-1 beta. These data demonstrate that human decidua can produce interleukin-1 in response to bacterial endotoxin.

Original languageEnglish
Pages (from-to)31-34
Number of pages4
JournalObstetrics and Gynecology
Volume73
Issue number1
StatePublished - Jan 1989
Externally publishedYes

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