Hepatic fatty acid profile in mice with nonalcoholic fatty liver disease using magnetic resonance spectroscopy

Aline Xavier*, Flavia Zacconi, Daniel Cabrera, Karen Fuenzalida, Marcelo Andia

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations


Nonalcoholic fatty liver disease (NAFLD) is characterized by the accumulation of intracellular fatty acids in the liver. The only method to confirm the stage of this disease is the biopsy, but it is invasive and risky to patients. The idea of defining a classifier using magnetic resonance spectroscopy (MRS) emerges due to the need to find a way to replace biopsy with a non-invasive method that can classify NAFLD based on the chemical structure of fatty acids stored in the liver. The purpose of this study is to investigate and compare the composition of fatty acids to the metabolites signals in MRS in NAFLD mice liver at 2 time-point during the progression of the disease. A group of C57BL/6 mice was fed with high-fat diet for one month (N = 8) and for three months (N = 6). First, we made a histological analysis to the liver. Then, we analysed the fatty acids with gas chromatography (GC) and MRS. As a result, the histological analysis showed the progression of fat content, and the GC analysis detected a different fatty acid liver composition during the progression of NAFLD along with an increase of the total fat storage in the liver. The differences in the composition fatty acids are also reflected in the MR Spectrum, which could have clinical potential for monitoring the progression of this disease with a non-invasive technique.

Original languageEnglish
Title of host publicationIFMBE Proceedings
EditorsRodrigo Costa-Felix, André Victor Alvarenga, João Carlos Machado
PublisherSpringer Verlag
Number of pages6
ISBN (Print)9789811321184
StatePublished - 2019
Externally publishedYes
Event26th Brazilian Congress on Biomedical Engineering, CBEB 2018 - Armação de Buzios, Brazil
Duration: 21 Oct 201825 Oct 2018

Publication series

NameIFMBE Proceedings
ISSN (Print)1680-0737
ISSN (Electronic)1433-9277


Conference26th Brazilian Congress on Biomedical Engineering, CBEB 2018
CityArmação de Buzios

Bibliographical note

Publisher Copyright:
© 2019, Springer Nature Singapore Pte Ltd.


  • Fatty acids
  • Magnetic resonance spectroscopy


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