Genetic variability of Coffea arabica L. accessions from Ethiopia evaluated with RAPDs

Alejandra Chaparro, M. A. Cristancho, H. A. Cortina, A. L. Gaitán

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The genetic diversity of 50 wild and semi-wild accessions of the Coffea arabica L. germplasm collection, gathered by the FAO and ORSTOM missions to Ethiopia, and maintained in Colombia by CENICAFE, was evaluated with RAPD markers. The evaluation was carried out in two phases: In phase one, the polymorphism of 8 Ethiopian accessions of different geographic origin, plus the cultivated variety 'Caturra' was assessed with the RAPD technique with forty-two 10-mer oligonucleotides. In phase two, 51 accessions were assessed with a set of 5 polymorphic primers that reproduced, with a correlation of 95%, the groups generated by the 24 polymorphic primers found in phase one. Principal Coordinate Analysis of molecular data revealed that a closely related group consisting of 86% of the Ethiopian C. arabica accessions evaluated are significantly different from the Caturra variety and could be used in a genetic breeding initiative to increase the variability of cultivated varieties. The results also indicate that a larger polymorphism is present in the Colombian replica of FAO Ethiopian coffee germplasm collection than previously reported.

Original languageEnglish
Pages (from-to)291-297
Number of pages7
JournalGenetic Resources and Crop Evolution
Volume51
Issue number3
DOIs
StatePublished - May 2004
Externally publishedYes

Bibliographical note

Funding Information:
The authors wish to thank the National Federation of Coffee Growers of Colombia and the Colombian Institute for the Development of Science and Technology ‘‘Francisco José de Caldas’’ (COLCIENCIAS), for their financial support for P. Chaparro, as well as to the staff of the Naranjal Experiment Station of CENICAFÉ, in particular to Mr. John Esteban Quin- tero for his cooperation in the collection of plant material.

Keywords

  • Caturra variety
  • Coffea arabica
  • Ethiopia
  • Genetic variability
  • RAPDs

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