TY - JOUR
T1 - Expression of proinflammatory cytokines and receptors by human fallopian tubes in organ culture following challenge with Neisseria gonorrhoeae
AU - Maisey, Kevin
AU - Nardocci, Gino
AU - Imarai, Monica
AU - Cardenas, Hugo
AU - Rios, Miguel
AU - Croxatto, Horacio B.
AU - Heckels, John E.
AU - Christodoulides, Myron
AU - Velasquez, Luis A.
PY - 2003/1/1
Y1 - 2003/1/1
N2 - Infection of the Fallopian tubes (FT) by Neisseria gonorrhoeae can lead to acute salpingitis, an inflammatory condition, which is a major cause of infertility. Challenge of explants of human FT with gonococci induced mRNA expression and protein secretion for the proinflammatory cytokines interleukin (IL)-1α, IL-1β, and tumor necrosis factor alpha (TNF-α) but not for granulocyte-macrophage colony-stimulating factor. In contrast, FT expression of IL-6 and of the cytokine receptors IL-6R, TNF receptor I (TNF-RI), and TNF-RII was constitutive and was not increased by gonococcal challenge. These studies suggest that several proinflammatory cytokines are likely to contribute to the cell and tissue damage observed in gonococcal salpingitis.
AB - Infection of the Fallopian tubes (FT) by Neisseria gonorrhoeae can lead to acute salpingitis, an inflammatory condition, which is a major cause of infertility. Challenge of explants of human FT with gonococci induced mRNA expression and protein secretion for the proinflammatory cytokines interleukin (IL)-1α, IL-1β, and tumor necrosis factor alpha (TNF-α) but not for granulocyte-macrophage colony-stimulating factor. In contrast, FT expression of IL-6 and of the cytokine receptors IL-6R, TNF receptor I (TNF-RI), and TNF-RII was constitutive and was not increased by gonococcal challenge. These studies suggest that several proinflammatory cytokines are likely to contribute to the cell and tissue damage observed in gonococcal salpingitis.
KW - Cytokines
KW - Fallopian Tubes
KW - Female
KW - Humans
KW - Inflammation
KW - Interleukins
KW - Microscopy, Confocal
KW - Neisseria gonorrhoeae
KW - Organ Culture Techniques
KW - Receptors, Cytokine
KW - Receptors, Interleukin-6
KW - Tumor Necrosis Factor-alpha
UR - http://www.scopus.com/inward/record.url?scp=0037222966&partnerID=8YFLogxK
U2 - 10.1128/IAI.71.1.527-532.2003
DO - 10.1128/IAI.71.1.527-532.2003
M3 - Article
C2 - 12496205
AN - SCOPUS:0037222966
SN - 0019-9567
VL - 71
SP - 527
EP - 532
JO - Infection and Immunity
JF - Infection and Immunity
IS - 1
ER -