Exploiting the natural poly(3-hydroxyalkanoates) production capacity of Antarctic Pseudomonas strains: from unique phenotypes to novel biopolymers

Nicolas Pacheco, Matias Orellana-Saez, Marzena Pepczynska, Javier Enrione, Monica Bassas-Galia, Jose M. Borrero-de Acuña, Flavia C. Zacconi, Andrés E. Marcoleta, Ignacio Poblete-Castro

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Extreme environments are a unique source of microorganisms encoding metabolic capacities that remain largely unexplored. In this work, we isolated two Antarctic bacterial strains able to produce poly(3-hydroxyalkanoates) (PHAs), which were classified after 16S rRNA analysis as Pseudomonas sp. MPC5 and MPC6. The MPC6 strain presented nearly the same specific growth rate whether subjected to a temperature of 4 °C 0.18 (1/h) or 30 °C 0.2 (1/h) on glycerol. Both Pseudomonas strains produced high levels of PHAs and exopolysaccharides from glycerol at 4 °C and 30 °C in batch cultures, an attribute that has not been previously described for bacteria of this genus. The MPC5 strain produced the distinctive medium-chain-length-PHA whereas Pseudomonas sp. MPC6 synthesized a novel polyoxoester composed of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate-co-3-hydroxydecanoate-co-3-hydroxydodecanoate). Batch bioreactor production of PHAs in MPC6 resulted in a titer of 2.6 (g/L) and 1.3 (g/L), accumulating 47.3% and 34.5% of the cell dry mass as PHA, at 30 and 4 °C, respectively. This study paves the way for using Antarctic Pseudomonas strains for biosynthesizing novel PHAs from low-cost substrates such as glycerol and the possibility to carry out the bioconversion process for biopolymer synthesis without the need for temperature control.
Original languageAmerican English
Pages (from-to)1139-1153
Number of pages15
JournalJournal of Industrial Microbiology and Biotechnology
Volume46
Issue number8
DOIs
StatePublished - 1 Aug 2019

Keywords

  • Antarctic Pseudomonas
  • Exopolysaccharide
  • Glycerol
  • Low temperature
  • Poly(3-hydroxyalkanoates)
  • Psychrophiles

Fingerprint Dive into the research topics of 'Exploiting the natural poly(3-hydroxyalkanoates) production capacity of Antarctic Pseudomonas strains: from unique phenotypes to novel biopolymers'. Together they form a unique fingerprint.

Cite this