Exosome-like vesicles in Apis mellifera bee pollen, honey and royal jelly contribute to their antibacterial and pro-regenerative activity

Christina M.A.P. Schuh, Sebastian Aguayo, Gabriela Zavala, Maroun Khoury

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Microvesicles are key players in cellular communication. As glandular secretions present a rich source of active exosomes, we hypothesized that exosome-like vesicles are present in Apis mellifera hypopharyngeal gland secretomal products (honey, royal jelly and bee pollen), and participate in their known antibacterial and pro-regenerative effects. We developed an isolation protocol based on serial centrifugation and ultracentrifugation steps and demonstrated the presence of protein-containing exosome-like vesicles in all three bee-derived products. Assessing their antibacterial properties, we found that exosome-like vesicles had bacteriostatic, bactericidal and biofilm-inhibiting effects on Staphylococcus aureus. Furthermore, we demonstrated that mesenchymal stem cells (MSCs) internalize bee-derived exosomelike vesicles and that these vesicles influence the migration potential of the MSCs. In an in vitro wound-healing assay, honey and royal jelly exosome-like vesicles increased migration of human MSCs, demonstrating their inter-kingdom activity. In summary, we have discovered exosome-like vesicles as a new, active compound in bee pollen, honey and royal jelly.

Original languageEnglish
Article numberjeb.208702
JournalJournal of Experimental Biology
Volume222
Issue number20
DOIs
StatePublished - 2019

Bibliographical note

Funding Information:
This work was supported by CONICYT FONDECYT (Comisión Nacional de Investigación Cientıf́ ica y Tecnológica – Fondo Nacional de Desarrollo Cientıf́ ico y Tecnológico) grant no. 11180406 and 11180101.

Publisher Copyright:
© 2019 Published by The Company of Biologists Ltd | Journal of Experimental Biology.

Keywords

  • Apis mellifera
  • Bactericide
  • Inter-kingdom communication
  • Intercellular communication
  • Microvesicle

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