Exhaled breath condensate analysis after long distance races

O. F. Araneda*, A. J. Guevara, C. Contreras, N. Lagos, F. J. Berral

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


The impact of an endurance race on pulmonary pro-oxidative formation and lipoperoxidation was evaluated using exhaled breath condensate (EBC). 3 groups of 12, 12 and 17 healthy recreational runners of both sexes ran 10, 21.1 and 42.2km, respectively. EBC samples were obtained before the run and at 20 and 80min post run. Concentrations of H2O2, NO2 -, malondialdehyde and pH were determined. The 10km group showed no post-run variations for H2O2 and NO2 - concentrations. The 21.1km group showed significant increments for NO2 -, and H2O2 concentrations in 20min and 80min samples. The 42.2km group, showed increased NO2 - concentration in 20min and 80min samples, while H2O 2 concentration increased only in the 20min sample. In the 10 and 42.2km groups neither malondialdehyde concentration nor pH showed differences. The 42.2km group exhibited ΔH2O2 and ΔNO 2 medians higher than the 10km group. ΔpH median decreased in 21.1 and 42.2km groups, exhibiting values significantly lower than the 10km group. ΔH2O2 y ΔNO2 - correlated directly with race time, while ΔpH, correlated inversely. In conclusion, intense prolonged exercise favors the increase in pulmonary pro-oxidative levels, with no modifications on lipoperoxidation. Running time relates to the magnitude of acute post exercise pro-oxidative formation.

Original languageEnglish
Pages (from-to)955-961
Number of pages7
JournalInternational Journal of Sports Medicine
Issue number12
StatePublished - 2012
Externally publishedYes


  • breath analysis
  • long distance runners
  • lung oxidative stress
  • physical exercise


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