Evaluation of mesenchymal stem cell proliferation using different low intensity pulsed ultrasound intensities

Daniela Becerra, Z. Jaime Díaz, A. Flavio Carrión, Carolina Inostroza, W. Rodrigo Oyonarte*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Mesenchymal Stem Cells (MSCs) can be affected in their capabilities to proliferate in vitro under physical and/or biochemical stimulation. The aim of this study was to select an optimal intensity level for low intensity pulsed ultrasound (LIPUS) stimulation of Sprague-Dawley bone marrow MSCs proliferation in vitro. Bone marrow MSCs of Sprague-Dawley rats where cultured (a-MEM, 20%FBS and 1%antibiotic) and stimulated with LIPUS (0,02 milisec), for 20 minutes twice daily for 10 days, at intensities of 0 (control), 30 and 50mW/cm2 (groups A, B, C). Cellular count and morphological evaluation were performed. ANOVA and Bonferroni tests were performed. LIPUS-stimulated cultures displayed greater cellular counts, and significant differences were observed between groups A and C (p<0,05). Morphological differences were observed between cells from LIPUS-stimulated and control groups. An intensity of 50mW/cm2 elicits increased cellular proliferation (p<0.05). Stimulation of MSCs cultures with LIPUS influences cellular morphology.

Translated title of the contributionEvaluation of mesenchymal stem cell proliferation using different low intensity pulsed ultrasound intensities
Original languageSpanish
Pages (from-to)621-626
Number of pages6
JournalInternational Journal of Morphology
Volume30
Issue number2
DOIs
StatePublished - Jun 2012

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