TY - JOUR
T1 - Evaluación de la proliferación de células madres mesenquimales estimuladas con diferentes intensidades de ultrasonido de baja intensidad
AU - Becerra, Daniela
AU - Jaime Díaz, Z.
AU - Flavio Carrión, A.
AU - Inostroza, Carolina
AU - Rodrigo Oyonarte, W.
PY - 2012/6
Y1 - 2012/6
N2 - Mesenchymal Stem Cells (MSCs) can be affected in their capabilities to proliferate in vitro under physical and/or biochemical stimulation. The aim of this study was to select an optimal intensity level for low intensity pulsed ultrasound (LIPUS) stimulation of Sprague-Dawley bone marrow MSCs proliferation in vitro. Bone marrow MSCs of Sprague-Dawley rats where cultured (a-MEM, 20%FBS and 1%antibiotic) and stimulated with LIPUS (0,02 milisec), for 20 minutes twice daily for 10 days, at intensities of 0 (control), 30 and 50mW/cm2 (groups A, B, C). Cellular count and morphological evaluation were performed. ANOVA and Bonferroni tests were performed. LIPUS-stimulated cultures displayed greater cellular counts, and significant differences were observed between groups A and C (p<0,05). Morphological differences were observed between cells from LIPUS-stimulated and control groups. An intensity of 50mW/cm2 elicits increased cellular proliferation (p<0.05). Stimulation of MSCs cultures with LIPUS influences cellular morphology.
AB - Mesenchymal Stem Cells (MSCs) can be affected in their capabilities to proliferate in vitro under physical and/or biochemical stimulation. The aim of this study was to select an optimal intensity level for low intensity pulsed ultrasound (LIPUS) stimulation of Sprague-Dawley bone marrow MSCs proliferation in vitro. Bone marrow MSCs of Sprague-Dawley rats where cultured (a-MEM, 20%FBS and 1%antibiotic) and stimulated with LIPUS (0,02 milisec), for 20 minutes twice daily for 10 days, at intensities of 0 (control), 30 and 50mW/cm2 (groups A, B, C). Cellular count and morphological evaluation were performed. ANOVA and Bonferroni tests were performed. LIPUS-stimulated cultures displayed greater cellular counts, and significant differences were observed between groups A and C (p<0,05). Morphological differences were observed between cells from LIPUS-stimulated and control groups. An intensity of 50mW/cm2 elicits increased cellular proliferation (p<0.05). Stimulation of MSCs cultures with LIPUS influences cellular morphology.
KW - Adult stem cells
KW - Mesenchymal stem cells
KW - Ultrasound
UR - http://www.scopus.com/inward/record.url?scp=84867537130&partnerID=8YFLogxK
U2 - 10.4067/s0717-95022012000200043
DO - 10.4067/s0717-95022012000200043
M3 - Article
AN - SCOPUS:84867537130
SN - 0717-9367
VL - 30
SP - 621
EP - 626
JO - International Journal of Morphology
JF - International Journal of Morphology
IS - 2
ER -