Evaluación de la Proliferación de Células Madres Mesenquimales Estimuladas con Diferentes Intensidades de Ultrasonido de Baja Intensidad

Daniela Becerra Giaverini; Z. Jaime Díaz; Flavio Carrión A; c silva; Rodrigo Oyonarte

doi:10.4067/s0717-95022012000200043

Evaluación de la Proliferación de Células Madres Mesenquimales Estimuladas con Diferentes Intensidades de Ultrasonido de Baja Intensidad

Translated title of the contribution: Evaluation of Mesenchymal Stem Cell Proliferation Using Different Low Intensity Pulsed Ultrasound Intensities

Daniela Becerra Giaverini, Z. Jaime Díaz, Flavio Carrión A, c silva, Rodrigo Oyonarte^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Mesenchymal Stem Cells (MSCs) can be affected in their capabilities to proliferate in vitro under physical and/or biochemical stimulation. The aim of this study was to select an optimal intensity level for low intensity pulsed ultrasound (LIPUS) stimulation of Sprague-Dawley bone marrow MSCs proliferation in vitro. Bone marrow MSCs of Sprague-Dawley rats where cultured (a-MEM, 20%FBS and 1%antibiotic) and stimulated with LIPUS (0,02 milisec), for 20 minutes twice daily for 10 days, at intensities of 0 (control), 30 and 50mW/cm2 (groups A, B, C). Cellular count and morphological evaluation were performed. ANOVA and Bonferroni tests were performed. LIPUS-stimulated cultures displayed greater cellular counts, and significant differences were observed between groups A and C (p<0,05). Morphological differences were observed between cells from LIPUS-stimulated and control groups. An intensity of 50mW/cm2 elicits increased cellular proliferation (p<0.05). Stimulation of MSCs cultures with LIPUS influences cellular morphology.

Translated title of the contribution	Evaluation of Mesenchymal Stem Cell Proliferation Using Different Low Intensity Pulsed Ultrasound Intensities
Original language	Spanish
Pages (from-to)	621-626
Number of pages	6
Journal	International Journal of Morphology
Volume	30
Issue number	2
DOIs	https://doi.org/10.4067/s0717-95022012000200043 https://doi.org/10.4067/S0717-95022012000200043
State	Published - Jun 2012

Keywords

Adult stem cells
Mesenchymal stem cells
ultrasound

Access to Document

Cite this

Becerra Giaverini, D., Jaime Díaz, Z., Carrión A, F., silva, C., & Oyonarte, R. (2012). Evaluación de la Proliferación de Células Madres Mesenquimales Estimuladas con Diferentes Intensidades de Ultrasonido de Baja Intensidad. International Journal of Morphology, 30(2), 621-626. https://doi.org/10.4067/s0717-95022012000200043, https://doi.org/10.4067/S0717-95022012000200043

@article{5f7c577e734540219a71cbfabbca824a,

title = "Evaluaci{\'o}n de la Proliferaci{\'o}n de C{\'e}lulas Madres Mesenquimales Estimuladas con Diferentes Intensidades de Ultrasonido de Baja Intensidad",

abstract = "Mesenchymal Stem Cells (MSCs) can be affected in their capabilities to proliferate in vitro under physical and/or biochemical stimulation. The aim of this study was to select an optimal intensity level for low intensity pulsed ultrasound (LIPUS) stimulation of Sprague-Dawley bone marrow MSCs proliferation in vitro. Bone marrow MSCs of Sprague-Dawley rats where cultured (a-MEM, 20%FBS and 1%antibiotic) and stimulated with LIPUS (0,02 milisec), for 20 minutes twice daily for 10 days, at intensities of 0 (control), 30 and 50mW/cm2 (groups A, B, C). Cellular count and morphological evaluation were performed. ANOVA and Bonferroni tests were performed. LIPUS-stimulated cultures displayed greater cellular counts, and significant differences were observed between groups A and C (p<0,05). Morphological differences were observed between cells from LIPUS-stimulated and control groups. An intensity of 50mW/cm2 elicits increased cellular proliferation (p<0.05). Stimulation of MSCs cultures with LIPUS influences cellular morphology.",

keywords = "C{\'e}lulas madre mesenquimales, ultrasonido, Adult stem cells, Mesenchymal stem cells, ultrasound",

author = "{Becerra Giaverini}, Daniela and {Jaime D{\'i}az}, Z. and {Carri{\'o}n A}, Flavio and c silva and Rodrigo Oyonarte",

year = "2012",

month = jun,

doi = "10.4067/s0717-95022012000200043",

language = "Spanish",

volume = "30",

pages = "621--626",

journal = "International Journal of Morphology",

issn = "0717-9367",

publisher = "Universidad de la Frontera",

number = "2",

}

Becerra Giaverini, D, Jaime Díaz, Z, Carrión A, F, silva, C & Oyonarte, R 2012, 'Evaluación de la Proliferación de Células Madres Mesenquimales Estimuladas con Diferentes Intensidades de Ultrasonido de Baja Intensidad', International Journal of Morphology, vol. 30, no. 2, pp. 621-626. https://doi.org/10.4067/s0717-95022012000200043, https://doi.org/10.4067/S0717-95022012000200043

TY - JOUR

T1 - Evaluación de la Proliferación de Células Madres Mesenquimales Estimuladas con Diferentes Intensidades de Ultrasonido de Baja Intensidad

AU - Becerra Giaverini, Daniela

AU - Jaime Díaz, Z.

AU - Carrión A, Flavio

AU - silva, c

AU - Oyonarte, Rodrigo

PY - 2012/6

Y1 - 2012/6

N2 - Mesenchymal Stem Cells (MSCs) can be affected in their capabilities to proliferate in vitro under physical and/or biochemical stimulation. The aim of this study was to select an optimal intensity level for low intensity pulsed ultrasound (LIPUS) stimulation of Sprague-Dawley bone marrow MSCs proliferation in vitro. Bone marrow MSCs of Sprague-Dawley rats where cultured (a-MEM, 20%FBS and 1%antibiotic) and stimulated with LIPUS (0,02 milisec), for 20 minutes twice daily for 10 days, at intensities of 0 (control), 30 and 50mW/cm2 (groups A, B, C). Cellular count and morphological evaluation were performed. ANOVA and Bonferroni tests were performed. LIPUS-stimulated cultures displayed greater cellular counts, and significant differences were observed between groups A and C (p<0,05). Morphological differences were observed between cells from LIPUS-stimulated and control groups. An intensity of 50mW/cm2 elicits increased cellular proliferation (p<0.05). Stimulation of MSCs cultures with LIPUS influences cellular morphology.

AB - Mesenchymal Stem Cells (MSCs) can be affected in their capabilities to proliferate in vitro under physical and/or biochemical stimulation. The aim of this study was to select an optimal intensity level for low intensity pulsed ultrasound (LIPUS) stimulation of Sprague-Dawley bone marrow MSCs proliferation in vitro. Bone marrow MSCs of Sprague-Dawley rats where cultured (a-MEM, 20%FBS and 1%antibiotic) and stimulated with LIPUS (0,02 milisec), for 20 minutes twice daily for 10 days, at intensities of 0 (control), 30 and 50mW/cm2 (groups A, B, C). Cellular count and morphological evaluation were performed. ANOVA and Bonferroni tests were performed. LIPUS-stimulated cultures displayed greater cellular counts, and significant differences were observed between groups A and C (p<0,05). Morphological differences were observed between cells from LIPUS-stimulated and control groups. An intensity of 50mW/cm2 elicits increased cellular proliferation (p<0.05). Stimulation of MSCs cultures with LIPUS influences cellular morphology.

KW - Células madre mesenquimales

KW - ultrasonido

KW - Adult stem cells

KW - Mesenchymal stem cells

KW - ultrasound

UR - http://www.scopus.com/inward/record.url?scp=84867537130&partnerID=8YFLogxK

U2 - 10.4067/s0717-95022012000200043

DO - 10.4067/s0717-95022012000200043

M3 - Article

AN - SCOPUS:84867537130

SN - 0717-9367

VL - 30

SP - 621

EP - 626

JO - International Journal of Morphology

JF - International Journal of Morphology

IS - 2

ER -

Evaluación de la Proliferación de Células Madres Mesenquimales Estimuladas con Diferentes Intensidades de Ultrasonido de Baja Intensidad

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this