Efficacy of copper sulphate on Candida albicans on heat-polymerized acrylic resin

Elisa Huerta, Daisy Gorup, Manuel Manríquez, Rodrigo Cruz Choappa, Peggy Vieille, Wilfredo González-Arriagada

Research output: Contribution to journalArticlepeer-review

Abstract

The ageing of population is increasing, and a great percentage of these patients wear removable prostheses, and can suffer denture stomatitis, a condition that has been associated with candidiasis. Aims: To evaluate in vitro the effectiveness of Copper Sulfate against Candida albicans in samples of heat-polymerized acrylic resin, compared to nystatin, sodium hypochlorite and chlorhexidine. Materials and Methods: Initially, the minimum inhibitory concentration (MIC) of copper sulfate for Candida albicans was determined by microdilution. Then, 54 resin samples were divided into 6 treatment groups corresponding to Nystatin 100.000 UI, Sodium Hypochlorite 0.5%, chlorhexidine 0.12%, Copper Sulfate 4.7µg/ml, Copper Sulfate 9.4µg/ml and physiological saline solution, in which samples were submerged for 6 hours. Resin samples were then washed and cultured on solid media at 37°C for 72 hours. The number of colony-forming units was determined using a Quebec colony counter. The statistical analysis was performed using the Kruskal-Wallis test and the Mann-Whitney U test. Results: Copper sulfate at a concentration of 9.4µg/ml presented a similar effectiveness as the other control products regarding the reduction in the number of colonies of Candida albicans post-treatment. Conclusion: T he e ffectiveness of copper sulfate against Candida albicans on acrylic resin was similar to that of nystatin, sodium hypochlorite and chlorhexidine

Translated title of the contributionEfficacy of copper sulphate on Candida albicans on heat-polymerized acrylic resin
Original languageSpanish
Pages (from-to)57-62
Number of pages6
JournalJournal of Oral Research
Volume9
Issue number1
DOIs
StatePublished - 2020
Externally publishedYes

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