Effects of sodium hypochlorite as dentin deproteinizing agent and aging media on bond strength of two conventional adhesives

Gabriel Nima, Vanessa Cavalli, Renata Bacelar-Sá, Gláucia M.B. Ambrosano, Marcelo Giannini*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


This study evaluated the effect of 10% sodium hypochlorite (NaOCl) as deproteinizing agent and storage media on bond strength (BS) of two etch-and-rinse adhesive systems to dentin. Twenty-eight sound extracted human third molars were divided in four groups (n = 7), according to dentin treatment (conventional etching or etching followed by 10% NaOCl application) and adhesive systems (GB—Gluma 2Bond and OS—One-Step). After dentin treatments and adhesive application, a composite block was built-up on dentin surface and teeth were serially sectioned to obtain bonded sticks specimens. The sticks were submitted to three aging conditions: (24H) 24 hr in water (immediate), (SH) 3 hr of NaOCl accelerated-aging or (1Y) 1 year of water storage. Afterward, submitted to microtensile bond strength test (μTBS), failure modes and adhesive interfaces analyzes. Data were analyzed by two-way analysis of variance (ANOVA) and Tukey's test (α =.05). Dentin deproteinization before bonding significantly reduced μTBS for GB-treated group (p <.05), regardless the aging conditions. Water storage for 1 year (1Y) and NaOCl accelerated-aging (SH) decreased μTBS for both adhesives. Yet, the groups stored in NaOCl (SH) exhibited the lowest BS results (p <.05). Bond strength of deproteinized dentin was dependent on the adhesive system composition and NaOCl accelerated-aging promoted decreased bond strength and further degradation than water storage for 1 year.

Original languageEnglish
Pages (from-to)186-195
Number of pages10
JournalMicroscopy Research and Technique
Issue number2
StatePublished - 1 Feb 2020
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2019 Wiley Periodicals, Inc.


  • bonding systems
  • deproteinized dentin
  • hybrid layer
  • sodium hypochlorite


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