TY - JOUR
T1 - Effect of experimental dentin etchants on dentin bond strength, metalloproteinase inhibition, and antibiofilm activity
AU - Sahadi, Beatriz Ometto
AU - Sebold, Maicon
AU - André, Carolina Bosso
AU - Nima, Gabriel
AU - dos Santos, Andressa
AU - Chiari, Marina Damasceno e.Souza de Carvalho
AU - Nascimento, Fábio Dupart
AU - Tersariol, Ivarne Luis dos Santos
AU - Giannini, Marcelo
N1 - Publisher Copyright:
© 2024 Elsevier Inc.
PY - 2024/4
Y1 - 2024/4
N2 - Objective: this study evaluated dentin microtensile bond strength (µTBS) and failure modes (at 24 h and one year), bonding interface regarding hybridization, surface morphology regarding demineralization, in situ metalloproteinase (MMP) activity, and antibacterial effect of three dentin etchants compared to 35% phosphoric acid (PA). Materials and Methods: The Adper Single Bond 2 adhesive (3 M Oral Care) was applied on moist dentin etched with PA (control) or on air-dried dentin etched with 3% aluminum nitrate + 2% oxalic acid (AN), 6.8% ferric oxalate + 10% citric acid (FO), or 10% citric acid (CA). The µTBS test used 40 human teeth (n = 10). Failure modes and surface morphology were analyzed by scanning electron microscopy (n = 3), while bonding interface morphology and MMP activity were evaluated by laser scanning confocal microscopy (n = 3). Antibacterial activity was evaluated against S. Mutans biofilm by means of viable cells count (CFU/mL). Results: PA presented the highest bond strengths regardless of aging time. PA, AN, and CA showed stable bond strengths after one year of storage. Adhesive and mixed failures were predominant in all groups. Thin hybrid layers with short resin tags were observed for the experimental etchants. The AN-based etchant was able to inhibit MMP activity. All tested etchants presented antibacterial activity against S. Mutans biofilm. Significance: This study suggests different dentin etchants capable of inhibiting MMP activity while also acting as cavity disinfectants.
AB - Objective: this study evaluated dentin microtensile bond strength (µTBS) and failure modes (at 24 h and one year), bonding interface regarding hybridization, surface morphology regarding demineralization, in situ metalloproteinase (MMP) activity, and antibacterial effect of three dentin etchants compared to 35% phosphoric acid (PA). Materials and Methods: The Adper Single Bond 2 adhesive (3 M Oral Care) was applied on moist dentin etched with PA (control) or on air-dried dentin etched with 3% aluminum nitrate + 2% oxalic acid (AN), 6.8% ferric oxalate + 10% citric acid (FO), or 10% citric acid (CA). The µTBS test used 40 human teeth (n = 10). Failure modes and surface morphology were analyzed by scanning electron microscopy (n = 3), while bonding interface morphology and MMP activity were evaluated by laser scanning confocal microscopy (n = 3). Antibacterial activity was evaluated against S. Mutans biofilm by means of viable cells count (CFU/mL). Results: PA presented the highest bond strengths regardless of aging time. PA, AN, and CA showed stable bond strengths after one year of storage. Adhesive and mixed failures were predominant in all groups. Thin hybrid layers with short resin tags were observed for the experimental etchants. The AN-based etchant was able to inhibit MMP activity. All tested etchants presented antibacterial activity against S. Mutans biofilm. Significance: This study suggests different dentin etchants capable of inhibiting MMP activity while also acting as cavity disinfectants.
KW - Aluminum nitrate
KW - Anti-bacterial agents
KW - Citric acid
KW - Dental etching
KW - Ferric oxalate
KW - Matrix metalloproteinase inhibitors
UR - http://www.scopus.com/inward/record.url?scp=85185568150&partnerID=8YFLogxK
U2 - 10.1016/j.dental.2024.02.017
DO - 10.1016/j.dental.2024.02.017
M3 - Article
AN - SCOPUS:85185568150
SN - 0109-5641
VL - 40
SP - e12-e23
JO - Dental Materials
JF - Dental Materials
IS - 4
ER -