Effect of exercise duration on pro-oxidants and pH in exhaled breath condensate in humans

M. Tuesta, M. Alvear, T. Carbonell, C. García, R. Guzmán-Venegas, O. F. Araneda*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Exercise promotes pulmonary oxidative imbalance. In this regard, some evidence has been obtained from the study of exhaled breath condensate (EBC) during urban races, in which the factors involved in the occurrence of this process are still not characterized. In this paper, under laboratory conditions, both the role of time of exercise on the generation of pro-oxidants (H2O2, NO2 ) and pH have been assessed in EBC of 16 under-trained subjects who completed three tests of cycloergometric exercise at low intensity (30 % of VO2 max) with a duration of 10, 30, and 90 min. Samples were obtained as follows: immediately before and at 80 min post exertion in each test. In the 90-min test, an increase in H2O2, NO2 concentration in EBC at 80 min post exertion with no changes in the pH was observed. Total O2 consumption and total ventilation weakly correlated with the changes in H2O2 and NO2 . In conclusion, the concentration of pro-oxidants in the EBC depends on the duration of the exercise when it is performed at low intensity under laboratory conditions.

Original languageEnglish
Pages (from-to)353-360
Number of pages8
JournalJournal of Physiology and Biochemistry
Issue number2
StatePublished - 1 Jun 2016

Bibliographical note

Funding Information:
We acknowledge Mr. Luis Pizarro Zúñiga, Miss. Paulina Silva, and Mr. Gustavo Gómez for their technical assistance. This study was funded by Fondo de Ayuda a la Investigación (FAI), Universidad de los Andes, Project INOGTO2013 and the National Fund for Scientific & Technological Development (FONDECYT), Project number 11130082 granted to O.F. Araneda.

Publisher Copyright:
© 2016, University of Navarra.


  • Exhaled breath condensate
  • Lung
  • Pro-oxidants
  • Time of exercise


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