Early pregnancy levels of gingival crevicular fluid matrix metalloproteinases-8 and −9 are associated with the severity of periodontitis and the development of gestational diabetes mellitus

Alejandra Chaparro, Ornella Realini, Marcela Hernández, Daniela Albers, Laura Weber, Valeria Ramírez, Fernanda Param, Juan Pedro Kusanovic, Timo Sorsa, Gregory Edward Rice, Sebastián E. Illanes

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3 Scopus citations

Abstract

Aim: To explore the association between the concentrations of matrix metalloproteinase (MMP)-8 and −9 in gingival crevicular fluid (GCF) during early pregnancy with the periodontal diagnosis and the risk of GDM development. Materials and methods: A prospective cohort study, including 314 women, enrolled at 11 to 14 weeks of pregnancy was conducted. A complete maternal/obstetric and periodontal exam was performed, and GCF samples were obtained for the MMP-8 and −9 determination by Multiplex Elisa Assays. Mann-Whitney test; Spearman's correlation and log-binomial regression model estimated the association between MMPs concentration in GCF and GDM. Results: Fourteen percent of the pregnancies were diagnosed with GDM. An increase in the concentration of MMP-8 and −9 in women with periodontitis stage III and IV compared to periodontitis stage I was observed (99.31 ng/mL [IQR: 85.32] versus 71.95 ng/mL [IQR: 54.04], and 262.4 ng/mL [IQR: 312.55] versus 114.1 ng/mL [IQR: 184.94], respectively). Women who developed GDM showed increased concentrations of MMP-8 and −9 in GCF since the beginning of pregnancy (P = 0.0381; P = 0.0302, respectively). MMP-8 concentration in GCF was associated with GDM (RR: 1.19; P = 0.045; CI 95% 1.00 to 1.40; and RR: 1.20; P = 0.063; CI 95% 0.99 to 1.45 in the adjusted model). Conclusion(s): GCF concentrations of MMP-8 and −9 at early of pregnancy are increased in women with severe periodontitis and associated with the GDM development.
Original languageAmerican English
JournalJournal of Periodontology
DOIs
StatePublished - 1 Jan 2020

Keywords

  • biomarkers
  • diabetes
  • gestational
  • gingival crevicular fluid
  • metalloproteinases
  • periodontitis

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