Early detection of cell-free fetal DNA in maternal plasma

S. Illanes; M. Denbow; C. Kailasam; K. Finning; P. W. Soothill

doi:10.1016/j.earlhumdev.2006.11.001

Early detection of cell-free fetal DNA in maternal plasma

S. Illanes^*, M. Denbow, C. Kailasam, K. Finning, P. W. Soothill

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

71 Scopus citations

Abstract

Objectives: We aimed to establish the earliest gestational age at which fetal DNA in maternal plasma could be detected and whether this was reliable at 12-13 weeks' gestation. Study design: A prospective observational cohort study of 32 pregnancies either after IVF or before prenatal diagnosis by CVS. Maternal blood was taken and RT-PCR was carried out to detect the multi-copy Y chromosome associated DSY14 gene. The end point was gender as assessed at delivery or on karyotype. Results: Y signal was obtained as early as 14 days post conception (4 weeks' gestation) and has a good prediction rate by 12 weeks' gestation. Conclusion: Free fetal DNA allows very early prediction of fetal sex in some cases and could be useful for clinical use for X-linked conditions by the end of the first trimester.

Original language	English
Pages (from-to)	563-566
Number of pages	4
Journal	Early Human Development
Volume	83
Issue number	9
DOIs	https://doi.org/10.1016/j.earlhumdev.2006.11.001
State	Published - Sep 2007
Externally published	Yes

Keywords

DYS14 gene
Earliest gestational age
Fetal DNA
Maternal plasma

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10.1016/j.earlhumdev.2006.11.001

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title = "Early detection of cell-free fetal DNA in maternal plasma",

abstract = "Objectives: We aimed to establish the earliest gestational age at which fetal DNA in maternal plasma could be detected and whether this was reliable at 12-13 weeks' gestation. Study design: A prospective observational cohort study of 32 pregnancies either after IVF or before prenatal diagnosis by CVS. Maternal blood was taken and RT-PCR was carried out to detect the multi-copy Y chromosome associated DSY14 gene. The end point was gender as assessed at delivery or on karyotype. Results: Y signal was obtained as early as 14 days post conception (4 weeks' gestation) and has a good prediction rate by 12 weeks' gestation. Conclusion: Free fetal DNA allows very early prediction of fetal sex in some cases and could be useful for clinical use for X-linked conditions by the end of the first trimester.",

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T1 - Early detection of cell-free fetal DNA in maternal plasma

AU - Illanes, S.

AU - Denbow, M.

AU - Kailasam, C.

AU - Finning, K.

AU - Soothill, P. W.

PY - 2007/9

Y1 - 2007/9

N2 - Objectives: We aimed to establish the earliest gestational age at which fetal DNA in maternal plasma could be detected and whether this was reliable at 12-13 weeks' gestation. Study design: A prospective observational cohort study of 32 pregnancies either after IVF or before prenatal diagnosis by CVS. Maternal blood was taken and RT-PCR was carried out to detect the multi-copy Y chromosome associated DSY14 gene. The end point was gender as assessed at delivery or on karyotype. Results: Y signal was obtained as early as 14 days post conception (4 weeks' gestation) and has a good prediction rate by 12 weeks' gestation. Conclusion: Free fetal DNA allows very early prediction of fetal sex in some cases and could be useful for clinical use for X-linked conditions by the end of the first trimester.

AB - Objectives: We aimed to establish the earliest gestational age at which fetal DNA in maternal plasma could be detected and whether this was reliable at 12-13 weeks' gestation. Study design: A prospective observational cohort study of 32 pregnancies either after IVF or before prenatal diagnosis by CVS. Maternal blood was taken and RT-PCR was carried out to detect the multi-copy Y chromosome associated DSY14 gene. The end point was gender as assessed at delivery or on karyotype. Results: Y signal was obtained as early as 14 days post conception (4 weeks' gestation) and has a good prediction rate by 12 weeks' gestation. Conclusion: Free fetal DNA allows very early prediction of fetal sex in some cases and could be useful for clinical use for X-linked conditions by the end of the first trimester.

KW - DYS14 gene

KW - Earliest gestational age

KW - Fetal DNA

KW - Maternal plasma

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Early detection of cell-free fetal DNA in maternal plasma

Abstract

Keywords

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