Directed Evolution of Enantioconvergency: the Case of an Epoxide Hydrolase-Catalyzed Reaction of a Racemic Epoxide

Huabao Zheng; Daniel Kahakeaw; Juan Pablo Acevedo; Manfred T. Reetz

doi:10.1002/cctc.201000122

Directed Evolution of Enantioconvergency: the Case of an Epoxide Hydrolase-Catalyzed Reaction of a Racemic Epoxide

Huabao Zheng, Daniel Kahakeaw, Juan Pablo Acevedo, Manfred T. Reetz^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Highly evolved: The Aspergillus niger epoxide hydrolase (ANEH) has been subjected to laboratory evolution with the creation of mutants that transform the racemic epoxide to a single stereoisomer with greater than 90 % conversion and 99 % enantiomeric excess.

Original language	English
Pages (from-to)	958-961
Number of pages	4
Journal	ChemCatChem
Volume	2
Issue number	8
DOIs	https://doi.org/10.1002/cctc.201000122
State	Published - 9 Aug 2010

Keywords

Alcohols
Directed evolution
Enantioselectivity
Enzymes
Hydrolases

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10.1002/cctc.201000122

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title = "Directed Evolution of Enantioconvergency: the Case of an Epoxide Hydrolase-Catalyzed Reaction of a Racemic Epoxide",

abstract = "Highly evolved: The Aspergillus niger epoxide hydrolase (ANEH) has been subjected to laboratory evolution with the creation of mutants that transform the racemic epoxide to a single stereoisomer with greater than 90 \% conversion and 99 \% enantiomeric excess.",

keywords = "Alcohols, Directed evolution, Enantioselectivity, Enzymes, Hydrolases",

author = "Huabao Zheng and Daniel Kahakeaw and Acevedo, \{Juan Pablo\} and Reetz, \{Manfred T.\}",

year = "2010",

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T2 - the Case of an Epoxide Hydrolase-Catalyzed Reaction of a Racemic Epoxide

AU - Zheng, Huabao

AU - Kahakeaw, Daniel

AU - Acevedo, Juan Pablo

AU - Reetz, Manfred T.

PY - 2010/8/9

Y1 - 2010/8/9

N2 - Highly evolved: The Aspergillus niger epoxide hydrolase (ANEH) has been subjected to laboratory evolution with the creation of mutants that transform the racemic epoxide to a single stereoisomer with greater than 90 % conversion and 99 % enantiomeric excess.

AB - Highly evolved: The Aspergillus niger epoxide hydrolase (ANEH) has been subjected to laboratory evolution with the creation of mutants that transform the racemic epoxide to a single stereoisomer with greater than 90 % conversion and 99 % enantiomeric excess.

KW - Alcohols

KW - Directed evolution

KW - Enantioselectivity

KW - Enzymes

KW - Hydrolases

UR - https://www.scopus.com/pages/publications/78149264575

U2 - 10.1002/cctc.201000122

DO - 10.1002/cctc.201000122

M3 - Article

AN - SCOPUS:78149264575

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VL - 2

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JO - ChemCatChem

JF - ChemCatChem

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Directed Evolution of Enantioconvergency: the Case of an Epoxide Hydrolase-Catalyzed Reaction of a Racemic Epoxide

Abstract

Keywords

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