Directed Evolution of Enantioconvergency: the Case of an Epoxide Hydrolase-Catalyzed Reaction of a Racemic Epoxide

Huabao Zheng; Daniel Kahakeaw; Juan Pablo Acevedo; Manfred T. Reetz

doi:10.1002/cctc.201000122

Directed Evolution of Enantioconvergency: the Case of an Epoxide Hydrolase-Catalyzed Reaction of a Racemic Epoxide

Huabao Zheng, Daniel Kahakeaw, Juan Pablo Acevedo, Manfred T. Reetz^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Highly evolved: The Aspergillus niger epoxide hydrolase (ANEH) has been subjected to laboratory evolution with the creation of mutants that transform the racemic epoxide to a single stereoisomer with greater than 90 % conversion and 99 % enantiomeric excess.

Original language	English
Pages (from-to)	958-961
Number of pages	4
Journal	ChemCatChem
Volume	2
Issue number	8
DOIs	https://doi.org/10.1002/cctc.201000122
State	Published - 9 Aug 2010

Keywords

Alcohols
Directed evolution
Enantioselectivity
Enzymes
Hydrolases

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10.1002/cctc.201000122

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abstract = "Highly evolved: The Aspergillus niger epoxide hydrolase (ANEH) has been subjected to laboratory evolution with the creation of mutants that transform the racemic epoxide to a single stereoisomer with greater than 90 % conversion and 99 % enantiomeric excess.",

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AU - Acevedo, Juan Pablo

AU - Reetz, Manfred T.

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AB - Highly evolved: The Aspergillus niger epoxide hydrolase (ANEH) has been subjected to laboratory evolution with the creation of mutants that transform the racemic epoxide to a single stereoisomer with greater than 90 % conversion and 99 % enantiomeric excess.

KW - Alcohols

KW - Directed evolution

KW - Enantioselectivity

KW - Enzymes

KW - Hydrolases

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Directed Evolution of Enantioconvergency: the Case of an Epoxide Hydrolase-Catalyzed Reaction of a Racemic Epoxide

Abstract

Keywords

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