Differential enrichment of TTF-I and Tip5 in the T-like promoter structures of the rDNA contribute to the epigenetic response of Cyprinus carpio during environmental adaptation

Gino Nardocci, Nicolas G. Simonet, Cristina Navarro, Gernot Längst, Marco Alvarez

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

To ensure homeostasis, ectothermic organisms adapt to environmental variations through molecular mechanisms. We previously reported that during the seasonal acclimatization of the common carp Cyprinus carpio, molecular and cellular functions are reprogrammed, resulting in distinctive traits. Importantly, the carp undergoes a drastic rearrangement of nucleolar components during adaptation. This ultrastructural feature reflects a fine modulation of rRNA gene transcription. Specifically, we identified the involvement of the transcription termination factor I (TTF-I) and Tip-5 (member of nucleolar remodeling complex, NoRC) in the control of rRNA transcription. Our results suggest that differential Tip5 enrichment is essential for silencing carp ribosomal genes and that the T0 element is key for regulating the ribosomal gene during the acclimatization process. Interestingly, the expression and content of Tip5 were significantly higher in winter than in summer. Since carp ribosomal gene expression is lower in the winter than in summer, and considering that expression concomitantly occurs with nucleolar ultrastructural changes of the acclimatization process, these results indicate that Tip5 importantly contributes to silencing the ribosomal genes. In conclusion, the current study provides novel evidence on the contributions of TTF-I and NoRC in the environmental reprogramming of ribosomal genes during the seasonal adaptation process in carp.

Original languageEnglish
Pages (from-to)315-321
Number of pages7
JournalBiochemistry and Cell Biology
Volume94
Issue number4
DOIs
StatePublished - 2016
Externally publishedYes

Bibliographical note

Funding Information:
This study was supported by the following grants: FONDECYT 1120873, CONICYT/FONDAP 15110027, DFG-Conicyt 2009-083, and DI-07-09/I from Universidad Andres Bello

Publisher Copyright:
© Published by NRC Research Press.

Keywords

  • Acclimatization
  • Epigenetics
  • Homeostasis
  • Nucleolar remodeling complex
  • Ribosomal genes

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