TY - JOUR
T1 - Diagnostic accuracy for apical and chronic periodontitis biomarkers in gingival crevicular fluid
T2 - An exploratory study
AU - Baeza, Mauricio
AU - Garrido, Mauricio
AU - Hernández-Ríos, Patricia
AU - Dezerega, Andrea
AU - García-Sesnich, Jocelyn
AU - Strauss, Franz
AU - Aitken, Juan Pablo
AU - Lesaffre, Emmanuel
AU - Vanbelle, Sophie
AU - Gamonal, Jorge
AU - Brignardello-Petersen, Romina
AU - Tervahartiala, Taina
AU - Sorsa, Timo
AU - Hernández, Marcela
N1 - Publisher Copyright:
© 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Aim The aim of this study was to assess the levels and diagnostic accuracy of a set of potential biomarkers of periodontal tissue metabolism in gingival crevicular fluid (GCF) from patients with chronic periodontitis (CP) and asymptomatic apical periodontitis (AAP). Materials and Methods Thirty one GCF samples from 11 CP patients, 44 GCF samples from 38 AAP patients and 31 GCF samples from 13 healthy volunteers were obtained (N = 106). Matrix metalloproteinases (MMPs) -2 and -9 were determined by zymography; levels of MMP-8 by ELISA and IFMA and MPO by ELISA. IL-1, IL-6, TNFα, DKK-1, Osteonectin, Periostin, TRAP-5 and OPG were determined by a multiplex quantitative panel. Statistical analysis was performed using linear mixed-effects models. Results The MMP-9 and MMP-8 were higher in CP, followed by AAP, versus healthy individuals (p < 0.05). ProMMP-2, MPO, IL-1, IL-6, PTN, TRAP-5 and OPG were significantly higher in CP when compared with AAP and healthy patients (p < 0.05). The highest diagnostic accuracies were observed for ProMMP-2, ProMMP-9, MMP-8 and TRAP-5 (AUC > 0.97) in CP, and for the active form of MMP-9 and MMP-8 (AUC > 0.90) in AAP. Conclusion Gingival crevicular fluid composition is modified by CP and AAP. MMP-9 and MMP-8 show diagnostic potential for CP and AAP, whereas MMP-2 and TRAP-5 are useful only for CP.
AB - Aim The aim of this study was to assess the levels and diagnostic accuracy of a set of potential biomarkers of periodontal tissue metabolism in gingival crevicular fluid (GCF) from patients with chronic periodontitis (CP) and asymptomatic apical periodontitis (AAP). Materials and Methods Thirty one GCF samples from 11 CP patients, 44 GCF samples from 38 AAP patients and 31 GCF samples from 13 healthy volunteers were obtained (N = 106). Matrix metalloproteinases (MMPs) -2 and -9 were determined by zymography; levels of MMP-8 by ELISA and IFMA and MPO by ELISA. IL-1, IL-6, TNFα, DKK-1, Osteonectin, Periostin, TRAP-5 and OPG were determined by a multiplex quantitative panel. Statistical analysis was performed using linear mixed-effects models. Results The MMP-9 and MMP-8 were higher in CP, followed by AAP, versus healthy individuals (p < 0.05). ProMMP-2, MPO, IL-1, IL-6, PTN, TRAP-5 and OPG were significantly higher in CP when compared with AAP and healthy patients (p < 0.05). The highest diagnostic accuracies were observed for ProMMP-2, ProMMP-9, MMP-8 and TRAP-5 (AUC > 0.97) in CP, and for the active form of MMP-9 and MMP-8 (AUC > 0.90) in AAP. Conclusion Gingival crevicular fluid composition is modified by CP and AAP. MMP-9 and MMP-8 show diagnostic potential for CP and AAP, whereas MMP-2 and TRAP-5 are useful only for CP.
KW - asymptomatic apical periodontitis
KW - biomarkers
KW - chronic periodontitis
KW - gingival crevicular fluid
KW - sensitivity
KW - specificity
UR - http://www.scopus.com/inward/record.url?scp=84959115004&partnerID=8YFLogxK
U2 - 10.1111/jcpe.12479
DO - 10.1111/jcpe.12479
M3 - Article
C2 - 26556177
AN - SCOPUS:84959115004
SN - 0303-6979
VL - 43
SP - 34
EP - 45
JO - Journal of Clinical Periodontology
JF - Journal of Clinical Periodontology
IS - 1
ER -