Diabetes alters the involvement of myofibroblasts during periodontal wound healing

Ignacio Retamal, Romina Hernández, Victoria Velarde, Alejandro Oyarzún, Constanza Martínez, María Julieta González, Jorge Martínez, Patricio C. Smith*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Objectives: Myofibroblasts constitute a specific cell phenotype involved in connective tissue healing. Diabetes alters the wound healing response. However, it is not clear whether diabetes modifies the involvement of myofibroblasts in periodontal wounds. Materials and Methods: Type I diabetes was induced in rats through streptozotocin injection, and periodontal wounds were performed. Wound healing was evaluated histologically at 2, 5, 7, and 15 days by measuring epithelial migration, neutrophil infiltration, and collagen and biofilm formation. Distribution of myofibroblasts was evaluated through immunofluorescence for α-smooth muscle actin. Data analyses were performed using the Shapiro–Wilk, ANOVA, or Kruskal–Wallis tests. Results: Diabetic wounds were characterized by delayed epithelial closure, increased neutrophil infiltration, biofilm formation, and reduced collagen formation. Quantification of the myofibroblasts showed a significant reduction at 5 and 7 days in wounds of diabetic rats and an increase at 15 days when compared to wounds of non-diabetic rats. Conclusions: Diabetic wound healing was associated with decreased epithelial and connective tissue healing, increased levels of inflammation, and biofilm formation. Myofibroblast differentiation was delayed in diabetic periodontal wounds at early time points. However, myofibroblasts persisted at later time points of healing. The present study suggests that diabetes alters the involvement of myofibroblasts during periodontal wound healing.

Original languageEnglish
Pages (from-to)1062-1071
Number of pages10
JournalOral Diseases
Volume26
Issue number5
DOIs
StatePublished - 1 Jul 2020

Bibliographical note

Funding Information:
We would like to thank Mrs. Paulina Zapata for histological processing and immunofluorescence of tissue samples. The present study was financed by a grant from the Chilean Fund for Science and Technology (FONDECYT grant 1170555 to PS).

Publisher Copyright:
© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved

Keywords

  • collagen
  • diabetes
  • fibroblast
  • myofibroblast
  • periodontal healing

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