TY - JOUR
T1 - Determinación del sexo fetal mediante adn libre fetal en plasma materno
AU - Sebastián Illanes, L.
AU - Paola Searovic, V.
AU - Karla Pino, L.
AU - Juan Trebilcock, G.
AU - Horacio Figueroa, D.
AU - Cristián Kottman, G.
AU - Juan Antonio Arraztoa, V.
PY - 2008
Y1 - 2008
N2 - Objective: To validate the use of cell free fetal DNA (ffDNA) in maternal plasma to predict fetal sex in the first half of pregnancy. Method: A prospective observational cohort study of 25 pregnancies between 5 and 15 weeks of gestation was studied (median gestational age of 9+1 weeks). Maternal blood was taken and q-PCR was carried out to detect the multi-copy Y chromosome associated DSY14 gene. The end point was gender as assessed by ultrasound at 20-25 weeks. Results: A Y signal was obtained in 12 patients, so a male fetus was predicted. The earliest signal was at 6+4 weeks. In 9 patients we didn't have any signal, so a female fetus was diagnosed. There were 4 cases where the criterion to define fetal sex was not fulfilled and were classified as equivocal (all of them before 8+3 weeks). The probability to predict fetal sex from the test was 84% (21 of 25 patients). However, when the diagnosis of fetal sex is made, there is a 100% correlation between the ultrasound and Q-PCR. Conclusion: Free fetal DNA in maternal plasma allows prediction of fetal sex in the first half of pregnancy.
AB - Objective: To validate the use of cell free fetal DNA (ffDNA) in maternal plasma to predict fetal sex in the first half of pregnancy. Method: A prospective observational cohort study of 25 pregnancies between 5 and 15 weeks of gestation was studied (median gestational age of 9+1 weeks). Maternal blood was taken and q-PCR was carried out to detect the multi-copy Y chromosome associated DSY14 gene. The end point was gender as assessed by ultrasound at 20-25 weeks. Results: A Y signal was obtained in 12 patients, so a male fetus was predicted. The earliest signal was at 6+4 weeks. In 9 patients we didn't have any signal, so a female fetus was diagnosed. There were 4 cases where the criterion to define fetal sex was not fulfilled and were classified as equivocal (all of them before 8+3 weeks). The probability to predict fetal sex from the test was 84% (21 of 25 patients). However, when the diagnosis of fetal sex is made, there is a 100% correlation between the ultrasound and Q-PCR. Conclusion: Free fetal DNA in maternal plasma allows prediction of fetal sex in the first half of pregnancy.
KW - Free fetal DNA
KW - Noninvasive prenatal diagnostic
KW - Real time PCR
UR - http://www.scopus.com/inward/record.url?scp=52949095416&partnerID=8YFLogxK
U2 - 10.4067/S0717-75262008000100005
DO - 10.4067/S0717-75262008000100005
M3 - Article
AN - SCOPUS:52949095416
SN - 0048-766X
VL - 73
SP - 27
EP - 30
JO - Revista Chilena de Obstetricia y Ginecologia
JF - Revista Chilena de Obstetricia y Ginecologia
IS - 1
ER -