Designing a gelatin/chitosan/hyaluronic acid biopolymer using a thermophysical approach for use in tissue engineering

Javier Enrione, Paulo Díaz-Calderón, Caroline R. Weinstein-Oppenheimer, Elizabeth Sánchez, Miguel A. Fuentes, Donald I. Brown, Hugo Herrera, Cristian A. Acevedo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


Cell culture on biopolymeric scaffolds has provided treatments for tissue engineering. Biopolymeric mixtures based on gelatin (Ge), chitosan (Ch) and hyaluronic acid (Ha) have been used to make scaffolds for wound healing. Thermal and physical properties of scaffolds prepared with Ge, Ch and Ha were characterized. Thermal characterization was made by using differential scanning calorimetry (DSC), and physical characterization by gas pycnometry and scanning electron microscopy. The effects of Ge content and cross-linking on thermophysical properties were evaluated by means of a factorial experiment design (central composite face centered). Gelatin content was the main factor that affects the thermophysical properties (microstructure and thermal transitions) of the scaffold. The effect of Ge content of the scaffolds for tissue engineering was studied by seeding skin cells on the biopolymers. The cell attachment was not significantly modified at different Ge contents; however, the cell growth rate increased linearly with the decrease of the Ge content. This relationship together with the thermophysical characterization may be used to design scaffolds for tissue engineering.

Original languageEnglish
Pages (from-to)1947-1956
Number of pages10
JournalBioprocess and Biosystems Engineering
Issue number12
StatePublished - Nov 2013


  • Biopolymeric scaffold
  • Chitosan
  • Gelatin
  • Hyaluronic acid
  • Thermophysical properties
  • Tissue engineering


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